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Literature summary extracted from

  • Papadovasilaki, M.; Oberthür, D.; Gessmann, R.; Sarrou, I.; Betzel, C.; Scoulica, E.Petratos, K.
    Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa (2015), Biochem. Biophys. Rep., 4, 152-157.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.47 expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.47 homology modeling of structure, and ternary complex with ATP and streptomycin or spectinomycin Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.47 additional information generation of a carboxy-terminal truncated variant molecule, residues 1-264. The truncated residues have little influence on protein folding, whereas they have a positive effect on the enzymic activity and stabilize dimers at high protein concentrations Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.47 kanamycin A competitive Pseudomonas aeruginosa
2.7.7.47 streptomycin strong substrate inhibition at 1mM MgCl2 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.47 0.0002
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.0004
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.0018
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.0033
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.0105
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.011
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.47 MgCl2 required, at least 5 mM Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.47 Pseudomonas aeruginosa Q9RGC2
-
-
2.7.7.47 Pseudomonas aeruginosa Ps100 Q9RGC2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.47 recombinant protein Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.47 1.3
-
wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.47 ATP + spectinomycin
-
Pseudomonas aeruginosa diphosphate + adenylylspectinomycin
-
?
2.7.7.47 ATP + spectinomycin
-
Pseudomonas aeruginosa Ps100 diphosphate + adenylylspectinomycin
-
?
2.7.7.47 ATP + streptomycin
-
Pseudomonas aeruginosa diphosphate + adenylylstreptomycin
-
?
2.7.7.47 ATP + streptomycin
-
Pseudomonas aeruginosa Ps100 diphosphate + adenylylstreptomycin
-
?
2.7.7.47 additional information no substrates: kanamycin A, gentamycin, and amikacin Pseudomonas aeruginosa ?
-
?
2.7.7.47 additional information no substrates: kanamycin A, gentamycin, and amikacin Pseudomonas aeruginosa Ps100 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.47 dimer and monomer, dynamic light scattering Pseudomonas aeruginosa
2.7.7.47 monomer and dimer, dynamic light scattering Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.7.47 AadA6
-
Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.47 49.5
-
melting temperature, wild-type Pseudomonas aeruginosa
2.7.7.47 51
-
melting temperature, C-terminal deletion mutant Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.47 0.26
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.41
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.47
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.61
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.63
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 0.66
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.47 39
-
ATP C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 55
-
ATP wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 140
-
streptomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 370
-
streptomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 1300
-
spectinomycin C-terminal deletion mutant, pH 7.5, 23°C Pseudomonas aeruginosa
2.7.7.47 1600
-
spectinomycin wild-type, pH 7.5, 23°C Pseudomonas aeruginosa