EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.47 | expression in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.47 | homology modeling of structure, and ternary complex with ATP and streptomycin or spectinomycin | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.47 | additional information | generation of a carboxy-terminal truncated variant molecule, residues 1-264. The truncated residues have little influence on protein folding, whereas they have a positive effect on the enzymic activity and stabilize dimers at high protein concentrations | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.47 | kanamycin A | competitive | Pseudomonas aeruginosa | |
2.7.7.47 | streptomycin | strong substrate inhibition at 1mM MgCl2 | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.47 | 0.0002 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.0004 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.0018 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.0033 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.0105 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.011 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.47 | MgCl2 | required, at least 5 mM | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.47 | Pseudomonas aeruginosa | Q9RGC2 | - |
- |
2.7.7.47 | Pseudomonas aeruginosa Ps100 | Q9RGC2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.47 | recombinant protein | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.47 | 1.3 | - |
wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.47 | ATP + spectinomycin | - |
Pseudomonas aeruginosa | diphosphate + adenylylspectinomycin | - |
? | |
2.7.7.47 | ATP + spectinomycin | - |
Pseudomonas aeruginosa Ps100 | diphosphate + adenylylspectinomycin | - |
? | |
2.7.7.47 | ATP + streptomycin | - |
Pseudomonas aeruginosa | diphosphate + adenylylstreptomycin | - |
? | |
2.7.7.47 | ATP + streptomycin | - |
Pseudomonas aeruginosa Ps100 | diphosphate + adenylylstreptomycin | - |
? | |
2.7.7.47 | additional information | no substrates: kanamycin A, gentamycin, and amikacin | Pseudomonas aeruginosa | ? | - |
? | |
2.7.7.47 | additional information | no substrates: kanamycin A, gentamycin, and amikacin | Pseudomonas aeruginosa Ps100 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.47 | dimer | and monomer, dynamic light scattering | Pseudomonas aeruginosa |
2.7.7.47 | monomer | and dimer, dynamic light scattering | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.47 | AadA6 | - |
Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.47 | 49.5 | - |
melting temperature, wild-type | Pseudomonas aeruginosa |
2.7.7.47 | 51 | - |
melting temperature, C-terminal deletion mutant | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.47 | 0.26 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.41 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.47 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.61 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.63 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 0.66 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.47 | 39 | - |
ATP | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 55 | - |
ATP | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 140 | - |
streptomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 370 | - |
streptomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 1300 | - |
spectinomycin | C-terminal deletion mutant, pH 7.5, 23°C | Pseudomonas aeruginosa | |
2.7.7.47 | 1600 | - |
spectinomycin | wild-type, pH 7.5, 23°C | Pseudomonas aeruginosa |