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Literature summary extracted from

  • Bezsudnova, E.Y.; Petrova, T.E.; Artemova, N.V.; Boyko, K.M.; Shabalin, I.G.; Rakitina, T.V.; Polyakov, K.M.; Popov, V.O.
    NADP-dependent aldehyde dehydrogenase from archaeon Pyrobaculum sp.1860: structural and functional features (2016), Archaea, 2016, 9127857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.4 expressed in Escherichia coli CodonPlus (DE3)-RIPL cells Pyrobaculum ferrireducens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.4 apoenzyme and in complex with isobutyraldehyde and/or NADP+, capillary counterdiffusion method Pyrobaculum ferrireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.4 15
-
 glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 20
-
 Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 21
-
 propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.4 Pyrobaculum ferrireducens G7VCG0
-
-
1.2.1.4 Pyrobaculum ferrireducens 1860 G7VCG0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.4 Source 30Q column chromatography and Superdex 200 gel filtration Pyrobaculum ferrireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.4 glyceraldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens glycerate + NADPH + H+
-
 ir
1.2.1.4 glyceraldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens 1860 glycerate + NADPH + H+
-
 ir
1.2.1.4 isobutyraldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens isobutyrate + NADPH + H+
-
 ir
1.2.1.4 isobutyraldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens 1860 isobutyrate + NADPH + H+
-
 ir
1.2.1.4 additional information no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde Pyrobaculum ferrireducens ?
-
 ?
1.2.1.4 additional information no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde Pyrobaculum ferrireducens 1860 ?
-
 ?
1.2.1.4 propionaldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens propanoate + NADPH + H+
-
 ir
1.2.1.4 propionaldehyde + NADP+ + H2O
-
 Pyrobaculum ferrireducens 1860 propanoate + NADPH + H+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.2.1.4 AlDHPyr1147
-
 Pyrobaculum ferrireducens
1.2.1.4 NADP-dependent aldehyde dehydrogenase
-
 Pyrobaculum ferrireducens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.4 75
-
-
 Pyrobaculum ferrireducens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.4 60 85
-
 Pyrobaculum ferrireducens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.4 1.2
-
 glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 2.9
-
 propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 4.1
-
 Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.4 8.5 9.5
-
 Pyrobaculum ferrireducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.4 NADP+ the enzyme is strictly specific for NADP+ Pyrobaculum ferrireducens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.4 0.08
-
 glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 0.14
-
 propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
1.2.1.4 0.2
-
 Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens