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Literature summary extracted from
- Purification and properties of thiosulfate reductase from Desulfovibrio gigas (1975), Arch. Microbiol., 105, 249-256.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.2.5 | enzyme loses activtiy upon freezing and thawing | Megalodesulfovibrio gigas |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.2.5 | 4-chloromercuribenzoate | 0.02 mM, 100% inhibition. Inhibition can be partially or completely reversed by cysteine | Megalodesulfovibrio gigas |  |
| 1.8.2.5 | Ag+ | 0.01 mM, 90% inhibition | Megalodesulfovibrio gigas | |
| 1.8.2.5 | Hg2+ | 0.01 mM, 100% inhibition | Megalodesulfovibrio gigas | |
| 1.8.2.5 | iodoacetate | 0.5 mM, 70% inhibition | Megalodesulfovibrio gigas | |
| 1.8.2.5 | N-ethylmaleimide | 0.5 mM, 55% inhibition | Megalodesulfovibrio gigas | |
| 1.8.2.5 | o-Iodosobenzoate | 0.05 mM, 52% inhibition | Megalodesulfovibrio gigas | |
| 1.8.2.5 | sulfite | 3 mM, 45% inhibition | Megalodesulfovibrio gigas | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.2.5 | 0.5 | - |
thiosulfate | 37°C, pH 7.6 | Megalodesulfovibrio gigas | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.5 | 220000 | - |
gel filtration | Megalodesulfovibrio gigas |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.2.5 | thiosulfate + 2 ferrocytochrome c3 | Megalodesulfovibrio gigas | - |
sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.2.5 | Megalodesulfovibrio gigas | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.2.5 | unstable during purification | Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.5 | 41 | - |
37°C, pH 7.6 | Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.2.5 | additional information | enzyme catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate. The most efficient coupling is obtained with a system containing cytochromes c3. The enzyme exhibits tetrathionate reductase activity. It does not show sulfite reductase activity | Megalodesulfovibrio gigas | ? | - |
? | |
| 1.8.2.5 | thiosulfate + 2 ferrocytochrome c3 | - |
Megalodesulfovibrio gigas | sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
| 1.8.2.5 | thiosulfate + reduced methyl viologen | - |
Megalodesulfovibrio gigas | sulfite + hydrogen sulfide + oxidized methyl viologen | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.5 | 7.4 | 8 | - |
Megalodesulfovibrio gigas |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.5 | 8.8 | - |
8% of maximum activity | Megalodesulfovibrio gigas |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.2.5 | cytochrome c3 | - |
Megalodesulfovibrio gigas |
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