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Literature summary extracted from

  • Graf, M.M.; Weber, S.; Kracher, D.; Kittl, R.; Sygmund, C.; Ludwig, R.; Peterbauer, C.; Haltrich, D.
    Characterization of three pyranose dehydrogenase isoforms from the litter-decomposing basidiomycete Leucoagaricus meleagris (syn. Agaricus meleagris) (2017), Appl. Microbiol. Biotechnol., 101, 2879-2891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Pichia pastoris strain X33 Leucoagaricus meleagris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.0103
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.0132
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.0201
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.0325
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.0791
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.109
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.161
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.53
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.64
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.66
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.82
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.95
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.38
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 1.88
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 2
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 2.22
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 2.39
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.28
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 9.34
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 10.3
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 11.6
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 17
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 18.3
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 29.7
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 34.9
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.1
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 54.6
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 62.5
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 76.7
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 94
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 113
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 154
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 181
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 185
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 235
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 2288
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 65000
-
x * 65000, SDS-PAGE Leucoagaricus meleagris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Leucoagaricus meleagris Q0R4L2 synonym Leucoagaricus meleagris
-
1.1.99.29 Leucoagaricus meleagris Q3L243 synonym Leucoagaricus meleagris
-
1.1.99.29 Leucoagaricus meleagris Q3L245 synonym Leucoagaricus meleagris
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein
-
Leucoagaricus meleagris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 ammonium sulfate precipitation, Ni2+-charged chelating Sepharose column chromatography, and DEAE Sepharose column chromatography Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 D-allose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-galactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
?
1.1.99.29 D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
?
1.1.99.29 D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
Leucoagaricus meleagris 3-dehydro-D-glucose + ?
-
?
1.1.99.29 D-glucose + ferricenium ion
-
Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-mannose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-xylose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 lactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 maltose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 maltotriose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 methyl-alpha-D-glucopyranoside + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 ? x * 65000, SDS-PAGE Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH1 isoform Leucoagaricus meleagris
1.1.99.29 PDH2 isoform Leucoagaricus meleagris
1.1.99.29 PDH3 isoform Leucoagaricus meleagris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.29 50
-
-
Leucoagaricus meleagris
1.1.99.29 52
-
-
Leucoagaricus meleagris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.29 66 73 isoform PDH1 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73.5°C and pH 5.0 Leucoagaricus meleagris
1.1.99.29 66 73 isoform PDH2 shows a significantly reduced thermostability over the whole pH range with a maximum melting temperature of 66.5°C at pH 6.0 Leucoagaricus meleagris
1.1.99.29 66 73 isoform PDH3 shows its highest thermal stability between pH values 5.0 and 6.0, with maximum melting temperature at 73°C and pH 6.0 Leucoagaricus meleagris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 0.48
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.57
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 4.28
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 5.32
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 7.57
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 7.63
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 8.16
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 9.86
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 16.3
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 18.4
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 19.3
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 21.2
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 23.4
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 26.5
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 29.5
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 33.7
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 34.3
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 34.7
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 35.3
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 35.7
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 35.9
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.2
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 41.5
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 42.2
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 44.7
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 48.1
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 51.3
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 65.4
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 75.3
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 85
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 109
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 130
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 137
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 146
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 209
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.29 9
-
-
Leucoagaricus meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD
-
Leucoagaricus meleagris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.29 0.0913
-
lactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.106
-
maltotriose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.188
-
D-mannose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.195
-
D-allose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.216
-
maltose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.282
-
D-mannose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.32
-
lactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.356
-
D-allose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.388
-
maltotriose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.439
-
lactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.574
-
maltotriose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.65
-
D-galactose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.658
-
maltose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.01
-
D-allose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.05
-
methyl-alpha-D-glucopyranoside isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.89
-
1,4-benzoquinone isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 2.48
-
D-glucose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.12
-
D-mannose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.16
-
D-xylose isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 3.41
-
D-galactose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 4.34
-
maltose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 8.59
-
D-xylose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 21.5
-
D-xylose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 38.9
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 42.5
-
methyl-alpha-D-glucopyranoside isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 47.4
-
1,4-benzoquinone isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 51.5
-
D-glucose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 53.6
-
D-galactose isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 62.9
-
methyl-alpha-D-glucopyranoside isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 67.4
-
D-glucose isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Leucoagaricus meleagris
1.1.99.29 94.6
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 532
-
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 780
-
1,4-benzoquinone isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 812
-
ferricenium ion isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 986
-
ferricenium ion isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 2400
-
ferricenium ion isoform PDH2, at pH 8.5 and 30°C Leucoagaricus meleagris