EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.13 | EDTA | 106.8% activity at 2 mM | Sphingobacterium faecium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.13 | Ag+ | 98.1% residual activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Ca2+ | 78.2% residual activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Cd2+ | 60.6% residual activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Cu2+ | 38.5% residual activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Fe2+ | 52.6% residual activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Hg2+ | complete inhibition at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Zn2+ | 91.1% residual activity at 2 mM | Sphingobacterium faecium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.13 | 1.1 | - |
D-glucose | at pH 6.0 and 25°C | Sphingobacterium faecium | |
1.1.99.13 | 1.7 | - |
sucrose | at pH 6.0 and 25°C | Sphingobacterium faecium | |
1.1.99.13 | 2.1 | - |
validoxylamine A | at pH 6.0 and 25°C | Sphingobacterium faecium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.13 | Ba2+ | 136.4% activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Co2+ | 116.7% activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Mg2+ | 107.3% activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | Mn2+ | 104.2% activity at 2 mM | Sphingobacterium faecium | |
1.1.99.13 | additional information | not influenced by K+ | Sphingobacterium faecium | |
1.1.99.13 | Ni2+ | 113.5% activity at 2 mM | Sphingobacterium faecium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.13 | 62300 | - |
- |
Sphingobacterium faecium |
1.1.99.13 | 63500 | - |
x * 63500, calculated from amino acid sequence | Sphingobacterium faecium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.13 | Sphingobacterium faecium | - |
ZJF-D6 CCTCC M 2013251 | - |
1.1.99.13 | Sphingobacterium faecium DSM 11690 | - |
ZJF-D6 CCTCC M 2013251 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.13 | High Q IEX column chromatography, hydroxyapatite column chromatography, and Sephadex G 75 gel filtration | Sphingobacterium faecium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.13 | 0.21 | - |
crude enzyme, at pH 6.0 and 25°C | Sphingobacterium faecium |
1.1.99.13 | 7.54 | - |
after 35.71fold purification, at pH 6.0 and 25°C | Sphingobacterium faecium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.13 | 2-deoxy-D-glucose + 2,6-dichlorophenolindophenol | 21.5% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-2-deoxy-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | alpha-methyl-D-glucoside + 2,6-dichlorophenolindophenol | 96% activity compared to D-glucose | Sphingobacterium faecium | methyl 3-dehydro-alpha-D-glucoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | cellobiose + 2,6-dichlorophenolindophenol | 97.4% activity compared to D-glucose | Sphingobacterium faecium | 4-O-beta-D-xylo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | D-fructose + 2,6-dichlorophenolindophenol | 10.8% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | D-galactose + 2,6-dichlorophenolindophenol | 63.5% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-D-galactose + 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | D-glucose + 2,6-dichlorophenolindophenol | - |
Sphingobacterium faecium | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | D-glucose + 2,6-dichlorophenolindophenol | - |
Sphingobacterium faecium DSM 11690 | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | D-xylose + 2,6-dichlorophenolindophenol | 3.1% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | D-xylose + 2,6-dichlorophenolindophenol | 3.1% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | ? | - |
? | |
1.1.99.13 | isomaltose + 2,6-dichlorophenolindophenol | 55.5% activity compared to D-glucose | Sphingobacterium faecium | 6-O-alpha-D-xylo-hexopyranosyl-4-ulose-alpha-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | L-arabinose + 2,6-dichlorophenolindophenol | 4.4% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | L-arabinose + 2,6-dichlorophenolindophenol | 4.4% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | ? | - |
? | |
1.1.99.13 | lactose + 2,6-dichlorophenolindophenol | 89.1% activity compared to D-glucose | Sphingobacterium faecium | 4-O-beta-D-xylo-hexopyranosyl-4-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | maltose + 2,6-dichlorophenolindophenol | 58% activity compared to D-glucose | Sphingobacterium faecium | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | maltose + 2,6-dichlorophenolindophenol | 58% activity compared to D-glucose | Sphingobacterium faecium DSM 11690 | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | additional information | enzyme activity is low with L-arabinose and D-xylose, and with 3-O-methyl-D-glucose, D-mannitol, D-mannose, sucralose and D-sorbitol giving no activity | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | additional information | enzyme activity is low with L-arabinose and D-xylose, and with 3-O-methyl-D-glucose, D-mannitol, D-mannose, sucralose and D-sorbitol giving no activity | Sphingobacterium faecium DSM 11690 | ? | - |
? | |
1.1.99.13 | sucrose + 2,6-dichlorophenolindophenol | - |
Sphingobacterium faecium | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | sucrose + 2,6-dichlorophenolindophenol | 46.5% activity compared to D-glucose | Sphingobacterium faecium | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.13 | trehalose + 2,6-dichlorophenolindophenol | 63.8% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | validamine + 2,6-dichlorophenolindophenol | 98.5% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | validamycin A + 2,6-dichlorophenolindophenol | 28.1% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | validoxylamine A + 2,6-dichlorophenolindophenol | 58.6% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? | |
1.1.99.13 | valienamine + 2,6-dichlorophenolindophenol | 4.4% activity compared to D-glucose | Sphingobacterium faecium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.13 | ? | x * 63500, calculated from amino acid sequence | Sphingobacterium faecium |
1.1.99.13 | ? | x * 62300, SDS-PAGE | Sphingobacterium faecium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.13 | G3DH | - |
Sphingobacterium faecium |
1.1.99.13 | glucoside 3-dehydrogenase | - |
Sphingobacterium faecium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.13 | 50 | 60 | the enzyme activity decreases significantly at 55 and 60°C, and 67.8% of enzyme activity is maintained when the enzyme is incubated at 50°C for 120 min | Sphingobacterium faecium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.13 | 6.2 | - |
- |
Sphingobacterium faecium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.13 | 5 | 6.6 | the enzyme remains stable at pH 5.0-6.6 for 24 h | Sphingobacterium faecium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.13 | FAD | - |
Sphingobacterium faecium |