EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | fructose 1,6-bisphosphate | required | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | fructose 1,6-bisphosphate | required | Pseudomonas aeruginosa | |
1.1.1.28 | additional information | no activation of the enzyme by fructose 1,6-bisphosphate and Mg2+ | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.28 | gene ecd_01352, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Escherichia coli |
1.1.1.28 | gene fn0511, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | gene pa0927, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | glyoxylate | - |
Pseudomonas aeruginosa | |
1.1.1.28 | Hydroxypyruvate | - |
Pseudomonas aeruginosa | |
1.1.1.28 | oxaloacetate | - |
Escherichia coli | |
1.1.1.28 | oxaloacetate | - |
Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | oxamate | constant inhibition at pH 7.0 and pH 8.0 | Escherichia coli | |
1.1.1.28 | oxamate | inhibits the catalytic reaction of FNLDH competitively with pyruvate, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | oxamate | inhibits the catalytic reaction of PALDH in a mixed--type manner at pH 7.0, but markedly enhances the enzyme reaction at low concentration through canceling of the apparent homotropic cooperativity at pH 8.0 | Pseudomonas aeruginosa | |
1.1.1.28 | phenylpyruvate | - |
Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | pyruvate | - |
Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5 | Pseudomonas aeruginosa | |
1.1.1.28 | additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | additional information | - |
additional information | kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5 | Escherichia coli | |
1.1.1.28 | 0.019 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 0.022 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.028 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.031 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.035 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 0.036 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.055 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 0.088 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 0.09 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 0.092 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 0.1 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 0.31 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.34 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.36 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 0.64 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 0.68 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 0.85 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
1.1.1.28 | 1.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 1.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 1.7 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 1.7 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 2.5 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 2.5 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 2.6 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 2.6 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 3 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 3.5 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 3.8 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 4 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 5.8 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 6.5 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 6.8 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 7.1 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 7.2 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 7.6 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 11 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 12 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 12 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 18 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 18 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 20 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 30 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 54 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | Mg2+ | activates | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | Mg2+ | activates | Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 37800 | - |
- |
Pseudomonas aeruginosa |
1.1.1.28 | 38500 | - |
- |
Escherichia coli |
1.1.1.28 | 40000 | - |
4 * 40000, about, sequence calculation | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | 120000 | - |
recombinant His-tagged enzyme, gel filtration | Pseudomonas aeruginosa |
1.1.1.28 | 150000 | - |
recombinant His-tagged enzyme, peak 2, gel filtration | Escherichia coli |
1.1.1.28 | 160000 | - |
recombinant His-tagged enzyme, gel filtration | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | 330000 | - |
recombinant His-tagged enzyme, peak 1, gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | Fusobacterium nucleatum subsp. nucleatum | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | Escherichia coli | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | Pseudomonas aeruginosa | - |
(R)-lactate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.28 | Escherichia coli | A0A140N893 | - |
- |
1.1.1.28 | Fusobacterium nucleatum subsp. nucleatum | Q8RG11 | strain JCM 8532 | - |
1.1.1.28 | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | Q8RG11 | strain JCM 8532 | - |
1.1.1.28 | Pseudomonas aeruginosa | Q9I530 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.28 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Pseudomonas aeruginosa |
1.1.1.28 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | (R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Pseudomonas aeruginosa | |
1.1.1.28 | (R)-lactate + NAD+ = pyruvate + NADH + H+ | allosteric mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | - |
Fusobacterium nucleatum subsp. nucleatum | pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | - |
Escherichia coli | pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | 2-oxobutyrate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxybutyrate + NAD+ | - |
r | |
1.1.1.28 | 2-oxobutyrate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxybutyrate + NAD+ | - |
r | |
1.1.1.28 | 2-oxobutyrate + NADH + H+ | - |
Escherichia coli | 2-hydroxybutyrate + NAD+ | - |
r | |
1.1.1.28 | 2-oxobutyrate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | 2-hydroxybutyrate + NAD+ | - |
r | |
1.1.1.28 | 2-oxocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxycaproate + NAD+ | - |
r | |
1.1.1.28 | 2-oxocaproate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxycaproate + NAD+ | - |
r | |
1.1.1.28 | 2-oxocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | 2-hydroxycaproate + NAD+ | - |
r | |
1.1.1.28 | 2-oxoisocaproate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyisocaproate + NAD+ | - |
r | |
1.1.1.28 | 2-oxoisocaproate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxyisocaproate + NAD+ | - |
r | |
1.1.1.28 | 2-oxoisovalerate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyisovalerate + NAD+ | - |
r | |
1.1.1.28 | 2-oxovalerate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | 2-hydroxyvalerate + NAD+ | - |
r | |
1.1.1.28 | 2-oxovalerate + NADH + H+ | - |
Pseudomonas aeruginosa | 2-hydroxyvalerate + NAD+ | - |
r | |
1.1.1.28 | glyoxylate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | hydroxyacetate + NAD+ | - |
r | |
1.1.1.28 | glyoxylate + NADH + H+ | - |
Pseudomonas aeruginosa | hydroxyacetate + NAD+ | - |
r | |
1.1.1.28 | glyoxylate + NADH + H+ | - |
Escherichia coli | hydroxyacetate + NAD+ | - |
r | |
1.1.1.28 | hydroxypyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | glycerate + NAD+ | - |
r | |
1.1.1.28 | hydroxypyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | glycerate + NAD+ | - |
r | |
1.1.1.28 | hydroxypyruvate + NADH + H+ | - |
Escherichia coli | glycerate + NAD+ | - |
r | |
1.1.1.28 | hydroxypyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | glycerate + NAD+ | - |
r | |
1.1.1.28 | additional information | the enzyme ECLDH exhibits high substrate specificity toward pyruvate. No or poor activity with 2-oxovalerate, 2-oxoisovalerate, 2-oxocaproate, 2-oxoisocaproate, phenylpyruvate, and hydroxyphenylpyruvate | Escherichia coli | ? | - |
? | |
1.1.1.28 | additional information | the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Fusobacterium nucleatum subsp. nucleatum | ? | - |
? | |
1.1.1.28 | additional information | the enzyme PALDH exhibits high substrate specificity toward pyruvate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Pseudomonas aeruginosa | ? | - |
? | |
1.1.1.28 | additional information | the enzyme FNLDH exhibits a broad substrate specificity toward hydrophobic 2-oxoacids such as 2-oxobutyrate and 2-oxovalerate. Poor activity with hydroxyphenylpyruvate and 2-oxoisovalerate | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | ? | - |
? | |
1.1.1.28 | oxaloacetate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | malate + NAD+ | - |
r | |
1.1.1.28 | oxaloacetate + NADH + H+ | - |
Pseudomonas aeruginosa | malate + NAD+ | - |
r | |
1.1.1.28 | oxaloacetate + NADH + H+ | - |
Escherichia coli | malate + NAD+ | - |
r | |
1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | D-phenyllactate + NAD+ | - |
r | |
1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | D-phenyllactate + NAD+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum | (R)-lactate + NAD+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Pseudomonas aeruginosa | (R)-lactate + NAD+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Escherichia coli | (R)-lactate + NAD+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 | (R)-lactate + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.28 | homooctamer | 8 * 38500, about, sequence calculation, the apparently octameric form of ECLDH is likely an artificial product due to the overexpression, and exhibits slightly but significantly lower specific activity than the tetrameric form | Escherichia coli |
1.1.1.28 | homotetramer | 4 * 40000, about, sequence calculation | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | homotetramer | 4 * 38500, about, sequence calculation | Escherichia coli |
1.1.1.28 | homotrimer | 4 * 37800, about, sequence calculation | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.28 | D-LDH | - |
Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | D-LDH | - |
Pseudomonas aeruginosa |
1.1.1.28 | D-LDH | - |
Escherichia coli |
1.1.1.28 | ECBD_2243 | - |
Escherichia coli |
1.1.1.28 | ECLDH | - |
Escherichia coli |
1.1.1.28 | FN0511 | - |
Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | FNLDH | - |
Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | PA0927 | - |
Pseudomonas aeruginosa |
1.1.1.28 | PALDH | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 30 | - |
assay at | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | 30 | - |
assay at | Pseudomonas aeruginosa |
1.1.1.28 | 30 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 39 | - |
purified recombinant His-tagged enzyme, pH 8.0, stable up to | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | 49 | - |
purified recombinant His-tagged enzyme, pH 8.0, stable up to | Escherichia coli |
1.1.1.28 | 57 | - |
purified recombinant His-tagged enzyme, pH 5.0, stable up to | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 11 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 18 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 31 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 39 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 50 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 69 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 80 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 80 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 80 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 80 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 87 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 90 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 98 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 100 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 100 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 100 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 110 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 120 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 120 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 140 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 140 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 160 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 160 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 200 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 230 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 240 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 240 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
1.1.1.28 | 270 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 290 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 310 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 370 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 380 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 400 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 400 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 410 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 410 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 420 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 640 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 690 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 710 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 730 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 880 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 1100 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 7 | 8 | assay at | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | 7 | 8 | assay at | Pseudomonas aeruginosa |
1.1.1.28 | 7 | 8 | assay at | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 4 | 8 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Pseudomonas aeruginosa |
1.1.1.28 | 5 | 10 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Escherichia coli |
1.1.1.28 | 5 | 8.5 | purified recombinant His-tagged enzyme, 30°C, 1 h, stable at | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | NAD+ | - |
Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | NAD+ | - |
Pseudomonas aeruginosa | |
1.1.1.28 | NAD+ | - |
Escherichia coli | |
1.1.1.28 | NADH | - |
Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | NADH | - |
Pseudomonas aeruginosa | |
1.1.1.28 | NADH | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | additional information | - |
additional information | inhibition kinetics | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
1.1.1.28 | additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
1.1.1.28 | 1.8 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 5 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 19 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 20 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 23 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 44 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 140 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.28 | evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Pseudomonas aeruginosa |
1.1.1.28 | evolution | although both types of enzymes, NAD-dependent D- and L-lactate dehydrogenases (D-LDH, EC 1.1.1.28; and L-LDH, EC 1.1.1.27) catalyze identical reactions except for the distinct chirality of the lactate product, they are evolutionarily distinct from each other. Allosteric mechanism of D-LDHs, overview | Escherichia coli |
1.1.1.28 | metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Fusobacterium nucleatum subsp. nucleatum |
1.1.1.28 | metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Pseudomonas aeruginosa |
1.1.1.28 | metabolism | the enzyme acts at the last step of the glycolytic pathway under anaerobic conditions | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 0.7 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 1 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 2.6 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 3.2 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 4.2 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 5.3 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 8.7 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 11 | - |
phenylpyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 18 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 21 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 27 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 31 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 35 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 35 | - |
2-oxoisovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 50 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 51 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 53 | - |
2-oxoisocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 60 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 61 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 65 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 87 | - |
2-oxocaproate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 130 | - |
glyoxylate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 140 | - |
Hydroxypyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 150 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 160 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 200 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 200 | - |
2-oxovalerate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 230 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 230 | - |
oxaloacetate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 280 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
1.1.1.28 | 440 | - |
2-oxobutyrate | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 820 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 2500 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 2800 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 3700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 4000 | - |
pyruvate | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 4400 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Fusobacterium nucleatum subsp. nucleatum | |
1.1.1.28 | 7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa | |
1.1.1.28 | 7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Pseudomonas aeruginosa | |
1.1.1.28 | 7700 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with Mg2+ | Escherichia coli | |
1.1.1.28 | 7800 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Pseudomonas aeruginosa | |
1.1.1.28 | 8000 | - |
NADH | pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate | Escherichia coli | |
1.1.1.28 | 8100 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 12000 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Escherichia coli | |
1.1.1.28 | 17000 | - |
NADH | pH 7.0, 30°C, recombinant enzyme | Pseudomonas aeruginosa |