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Literature summary extracted from

  • Yonaha, K.; Toyama, S.
    Diamine transamination catalyzed by omega-amino acid:pyruvate aminotransferase of Pseudomonas sp. f-126 (1979), Agric. Biol. Chem., 43, 1043-1048.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.113 pyridoxal 5'-phosphate preincubation with pyridoxal phosphate leads to an increase in the enzyme activity and the protection of the activity against heat inactivation at 40-60 and 65-80°C, respectively Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.113 3-methyl-2-benzothiazolone hydrazone
-
 Pseudomonas sp.
2.6.1.113 aminoxy acetate
-
 Pseudomonas sp.
2.6.1.113 D-cycloserine
-
 Pseudomonas sp.
2.6.1.113 hydroxylamine
-
 Pseudomonas sp.
2.6.1.113 phenylhydrazine
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.113 0.00625
-
 pyruvate pH 10.0, temperature not specified in the publication Pseudomonas sp.
2.6.1.113 0.0769
-
 putrescine pH 10.0, temperature not specified in the publication Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.113 1,2-diaminoethane + pyruvate Pseudomonas sp. 38% of the activity with beta-alanine aminoethane + alanine
-
 ?
2.6.1.113 1,2-diaminoethane + pyruvate Pseudomonas sp. F-126 38% of the activity with beta-alanine aminoethane + alanine
-
 ?
2.6.1.113 1,6-diaminohexane + pyruvate Pseudomonas sp. 87% of the activity with beta-alanine 1-aminohexane + alanine
-
 ?
2.6.1.113 1,6-diaminohexane + pyruvate Pseudomonas sp. F-126 87% of the activity with beta-alanine 1-aminohexane + alanine
-
 ?
2.6.1.113 1,7-diaminoheptane + pyruvate Pseudomonas sp. 92% of the activity with beta-alanine 1-aminoheptane + alanine
-
 ?
2.6.1.113 1,7-diaminoheptane + pyruvate Pseudomonas sp. F-126 92% of the activity with beta-alanine 1-aminoheptane + alanine
-
 ?
2.6.1.113 1,8-diaminooctane + pyruvate Pseudomonas sp. 55% of the activity with beta-alanine 1-aminooctane + alanine
-
 ?
2.6.1.113 1,8-diaminooctane + pyruvate Pseudomonas sp. F-126 55% of the activity with beta-alanine 1-aminooctane + alanine
-
 ?
2.6.1.113 beta-alanine + pyruvate Pseudomonas sp. best substrate 3-oxopropionate + alanine
-
 ?
2.6.1.113 cadaverine + pyruvate Pseudomonas sp. 12% of the activity with beta-alanine 1-aminopentane + alanine
-
 ?
2.6.1.113 additional information Pseudomonas sp. one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane ?
-
 ?
2.6.1.113 additional information Pseudomonas sp. F-126 one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane ?
-
 ?
2.6.1.113 putrescine + pyruvate Pseudomonas sp. 55% of the activity with beta-alanine 4-aminobutanal + alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.113 Pseudomonas sp.
-
-
-
2.6.1.113 Pseudomonas sp. F-126
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.113
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.113 1,2-diaminoethane + pyruvate 38% of the activity with beta-alanine Pseudomonas sp. aminoethane + alanine
-
 ?
2.6.1.113 1,2-diaminoethane + pyruvate 38% of the activity with beta-alanine Pseudomonas sp. F-126 aminoethane + alanine
-
 ?
2.6.1.113 1,6-diaminohexane + pyruvate 87% of the activity with beta-alanine Pseudomonas sp. 1-aminohexane + alanine
-
 ?
2.6.1.113 1,6-diaminohexane + pyruvate 87% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminohexane + alanine
-
 ?
2.6.1.113 1,7-diaminoheptane + pyruvate 92% of the activity with beta-alanine Pseudomonas sp. 1-aminoheptane + alanine
-
 ?
2.6.1.113 1,7-diaminoheptane + pyruvate 92% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminoheptane + alanine
-
 ?
2.6.1.113 1,8-diaminooctane + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. 1-aminooctane + alanine
-
 ?
2.6.1.113 1,8-diaminooctane + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminooctane + alanine
-
 ?
2.6.1.113 beta-alanine + pyruvate best substrate Pseudomonas sp. 3-oxopropionate + alanine
-
 ?
2.6.1.113 cadaverine + pyruvate 12% of the activity with beta-alanine Pseudomonas sp. 1-aminopentane + alanine
-
 ?
2.6.1.113 additional information one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane Pseudomonas sp. ?
-
 ?
2.6.1.113 additional information one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane Pseudomonas sp. F-126 ?
-
 ?
2.6.1.113 putrescine + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. 4-aminobutanal + alanine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.113 60
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.113 60 65 in presence of pyridoxal 5'-phosphate, inactivation is delayed Pseudomonas sp.
2.6.1.113 70
-
 rapid inactivation above Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.113 10
-
 assay at Pseudomonas sp.
2.6.1.113 10.5 11
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.113 8 10 stable within Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.113 pyridoxal 5'-phosphate
-
 Pseudomonas sp.

Expression

EC Number Organism Comment Expression
2.6.1.113 Pseudomonas sp. expression is induced in presence of beta-alanine up