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Literature summary extracted from

  • Hu, X.Q.; Guo, P.C.; Ma, J.D.; Li, W.F.
    Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition (2013), Acta Crystallogr. Sect. F, 69, 1190-1195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.117 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.117 apo and NADPH-complexed enzyme forms, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, 0.1 M HEPES pH 7.5 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.117 D-arabinose + NADP+ Saccharomyces cerevisiae
-
 D-arabinono-1,4-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.117 Saccharomyces cerevisiae P38115
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.117 Ni-NTA column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.117 D-arabinose + NADP+
-
 Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.117 homodimer x-ray crystallography Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.117 ARA1
-
 Saccharomyces cerevisiae
1.1.1.117 D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.117 NADPH
-
 Saccharomyces cerevisiae