BRENDA - Enzyme Database

Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis

Harada, J.; Wada, K.; Yamaguchi, H.; Oh-oka, H.; Tamiaki, H.; Fukuyama, K.; Acta Crystallogr. Sect. F 61, 712-714 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.333
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.1.1.333
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl pH 8.5, and 12% (v/v) glycerol
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.333
S-adenosyl-L-methionine + a bacteriochlorophyllide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.333
Chlorobaculum tepidum
Q8KGE0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.333
Ni2+-affinity resin column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.333
S-adenosyl-L-methionine + a bacteriochlorophyllide d
-
739800
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.333
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.333
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum tepidum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.333
S-adenosyl-L-methionine
-
Chlorobaculum tepidum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.1.1.333
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM Tris-HCl pH 8.5, and 12% (v/v) glycerol
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.333
S-adenosyl-L-methionine + a bacteriochlorophyllide d
Chlorobaculum tepidum
-
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.333
Ni2+-affinity resin column chromatography and Sephacryl S-200 gel filtration
Chlorobaculum tepidum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.333
S-adenosyl-L-methionine + a bacteriochlorophyllide d
-
739800
Chlorobaculum tepidum
S-adenosyl-L-homocysteine + a bacteriochlorophyllide c
-
-
-
?