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Literature summary extracted from

  • Wang, L.; Peng, R.; Tian, Y.; Han, J.; Zhao, W.; Wang, B.; Liu, M.; Yao, Q.
    Characterization of a class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate from Sinorhizobium fredii (2014), World J. Microbiol. Biotechnol., 30, 2967-2973.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium fredii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 Al3+ 18.3% residual activity at 1 mM Sinorhizobium fredii
2.5.1.19 Ca2+ 90.1% residual activity at 1 mM Sinorhizobium fredii
2.5.1.19 Cu2+ 57.6% residual activity at 1 mM Sinorhizobium fredii
2.5.1.19 Fe2+ 25.2% residual activity at 1 mM Sinorhizobium fredii
2.5.1.19 Mn2+ 84% residual activity at 1 mM Sinorhizobium fredii
2.5.1.19 additional information insensitive to inhibition by glyphosate exhibiting Ki and IC50 values of 1.2 and 5.2 mM, respectively; not inhibited by EDTA Sinorhizobium fredii
2.5.1.19 Zn2+ 15.9% residual activity at 1 mM Sinorhizobium fredii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.19 K+ 194.2% activity at 100 mM Sinorhizobium fredii
2.5.1.19 Li+ 123.1% activity at 100 mM Sinorhizobium fredii
2.5.1.19 Na+ 156.6% activity at 100 mM Sinorhizobium fredii
2.5.1.19 NH4+ 235.8% activity at 100 mM Sinorhizobium fredii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 47000
-
 x * 47000, SDS-PAGE Sinorhizobium fredii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Sinorhizobium fredii
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Sinorhizobium fredii NGR234
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Sinorhizobium fredii C3MC80
-
-
2.5.1.19 Sinorhizobium fredii NGR234 C3MC80
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 Ni2+-NTA agarose column chromatography, and gel filtration Sinorhizobium fredii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Sinorhizobium fredii phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Sinorhizobium fredii NGR234 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? x * 47000, SDS-PAGE Sinorhizobium fredii

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enol-pyruvylshikimate-3-phosphate synthase
-
 Sinorhizobium fredii
2.5.1.19 AroA
-
 Sinorhizobium fredii
2.5.1.19 EPSP synhthase
-
 Sinorhizobium fredii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 50
-
-
 Sinorhizobium fredii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.19 40 60 more than 40% activity between 40 and 60°C Sinorhizobium fredii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.19 30 60 the enzyme has half-lives of 346, 131, 39 and 12 min at 30, 40, 50 and 60°C, respectively Sinorhizobium fredii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 8
-
-
 Sinorhizobium fredii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.19 6 9 more than 40% activity between pH 6.0 and 9.0 Sinorhizobium fredii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.19 5 9 the enzyme is stable over a broad range of pH 5.0-9.0, especially at pH 7.5, with more than 80% activity after 1 h incubation Sinorhizobium fredii