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Literature summary extracted from

  • Tomoike, F.; Nakagawa, N.; Kuramitsu, S.; Masui, R.
    Structural and biochemical studies on the reaction mechanism of uridine-cytidine kinase (2015), Protein J., 34, 411-420.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.48
-
 Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.48 in complex with cytidine and beta,gamma-methyleneadenosine 5'-triphosphate, sitting drop vapor diffusion method, using 0.1 M NaCl, 0.1 M bicine, pH 9.0, and 20% (w/v) polyethylene glycol monomethyl ether 550 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + uridine Thermus thermophilus
-
 ADP + UMP
-
 ?
2.7.1.48 ATP + uridine Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Thermus thermophilus Q5SKR5
-
-
2.7.1.48 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SKR5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 ammonium sulfate precipitation, Toyopearl Phenyl-650 M column chromatography, Toyopearl SP-650 column chromatography and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + uridine
-
 Thermus thermophilus ADP + UMP
-
 ?
2.7.1.48 ATP + uridine
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ADP + UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.48 UCK
-
 Thermus thermophilus
2.7.1.48 uridine-cytidine kinase
-
 Thermus thermophilus