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Literature summary extracted from

  • Hover, B.M.; Tonthat, N.K.; Schumacher, M.A.; Yokoyama, K.
    Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis (2015), Proc. Natl. Acad. Sci. USA, 112, 6347-6352.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.17 D128A less than 1% of wild-type activity Escherichia coli
4.6.1.17 D17A about 30% of wild-type activity Escherichia coli
4.6.1.17 E112A less than 1% of wild-type activity Escherichia coli
4.6.1.17 E114A less than 1% of wild-type activity Escherichia coli
4.6.1.17 H77A less than 1% of wild-type activity Escherichia coli
4.6.1.17 K131A less than 1% of wild-type activity Escherichia coli
4.6.1.17 K21A about 18% of wild-type activity Escherichia coli
4.6.1.17 K51A less than 1% of wild-type activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.17 0.00021
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.00025
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.001
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant K51A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.0016
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant H77A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.0024
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E112A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.0043
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E114A, pH not specified in the publication, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.22 Staphylococcus aureus P69848
-
-
4.1.99.22 Staphylococcus aureus NCTC 8325 P69848
-
-
4.6.1.17 Escherichia coli P0A738
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
Staphylococcus aureus (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor pyranopterin triphosphate is formed as a by-product, the amount is 0.4–3% of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine formed ?
4.1.99.22 GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
Staphylococcus aureus NCTC 8325 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor pyranopterin triphosphate is formed as a by-product, the amount is 0.4–3% of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine formed ?
4.6.1.17 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate
-
Escherichia coli cyclic pyranopterin phosphate + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.17 cyclic pyranopterin monophosphate synthase accessory protein
-
Escherichia coli
4.6.1.17 MoaC
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6.1.17 0.00002
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant K51A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.00004
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E114A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.00004
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant H77A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.00005
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E112A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.0088
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
4.6.1.17 0.0093
-
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli

General Information

EC Number General Information Comment Organism
4.1.99.22 physiological function the physiological function of MoaA is the conversion of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (GTP 3?,8-cyclase), and that of MoaC is to catalyze the rearrangement of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate into cyclic pyranopterin Staphylococcus aureus
4.6.1.17 physiological function the physiological function of MoaA is the conversion of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (GTP 3?,8-cyclase), and that of MoaC is to catalyze the rearrangement of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate into cyclic pyranopterin Escherichia coli