BRENDA - Enzyme Database show

In vitro reconstitution of indolmycin biosynthesis reveals the molecular basis of oxazolinone assembly

Du, Y.; Alkhalaf, L.; Ryan, K.; Proc. Natl. Acad. Sci. USA 112, 2717-2722 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.397
expressed in Escherichia coli BW25113 cells
Streptomyces griseus subsp. griseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus ATCC 12648
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
2.1.1.328
S-adenosyl-L-methionine + N-demethylindolmycin
Streptomyces griseus subsp. griseus
-
S-adenosyl-L-homocysteine + indolmycin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.397
Streptomyces griseus subsp. griseus
A0A0D4BSN8
-
-
1.1.1.397
Streptomyces griseus subsp. griseus ATCC 12648
A0A0D4BSN8
-
-
2.1.1.328
Streptomyces griseus subsp. griseus
A0A0D4BSP3
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus ATCC 12648
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
2.1.1.328
S-adenosyl-L-methionine + N-demethylindolmycin
-
739549
Streptomyces griseus subsp. griseus
S-adenosyl-L-homocysteine + indolmycin
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.328
S-adenosyl-L-methionine
-
Streptomyces griseus subsp. griseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.397
expressed in Escherichia coli BW25113 cells
Streptomyces griseus subsp. griseus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.328
S-adenosyl-L-methionine
-
Streptomyces griseus subsp. griseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus ATCC 12648
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
2.1.1.328
S-adenosyl-L-methionine + N-demethylindolmycin
Streptomyces griseus subsp. griseus
-
S-adenosyl-L-homocysteine + indolmycin
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
1.1.1.397
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus ATCC 12648
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
2.1.1.328
S-adenosyl-L-methionine + N-demethylindolmycin
-
739549
Streptomyces griseus subsp. griseus
S-adenosyl-L-homocysteine + indolmycin
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.1.1.328
physiological function
methyltransferase Ind7 is responsible for the last N-methyltransfer reaction to generate indolmycin
Streptomyces griseus subsp. griseus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.328
physiological function
methyltransferase Ind7 is responsible for the last N-methyltransfer reaction to generate indolmycin
Streptomyces griseus subsp. griseus