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Literature summary extracted from

  • Kang, J.Y.; Lim, S.J.; Kwon, O.; Lee, S.G.; Kim, H.H.; Oh, D.B.
    Enhanced bacterial alpha(2,6)-sialyltransferase reaction through an inhibition of its inherent sialidase activity by dephosphorylation of cytidine-5'-monophosphate (2015), PLoS ONE, 10, e0133739.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 CMP
-
 Photobacterium sp.
2.4.3.1 CMP
-
 Photobacterium leiognathi
2.4.3.1 CMP
-
 Photobacterium damselae
2.4.3.1 CMP-N-acetylneuraminate
-
 Photobacterium sp.
2.4.3.1 CMP-N-acetylneuraminate
-
 Photobacterium leiognathi
2.4.3.1 CMP-N-acetylneuraminate
-
 Photobacterium damselae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expressed in Escherichia coli BL21 cells Photobacterium sp.
2.4.3.1 expressed in Escherichia coli BL21 cells Photobacterium leiognathi
2.4.3.1 expressed in Escherichia coli BL21 cells Photobacterium damselae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 Alkaline phosphatase
-
 Photobacterium damselae
2.4.3.1 Alkaline phosphatase
-
 Photobacterium leiognathi
2.4.3.1 Alkaline phosphatase
-
 Photobacterium sp.
2.4.3.1 cytidine
-
 Photobacterium damselae
2.4.3.1 cytidine
-
 Photobacterium leiognathi
2.4.3.1 cytidine
-
 Photobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.0047
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium sp.
2.4.3.1 0.0278
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium leiognathi
2.4.3.1 0.0361
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium damselae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium sp.
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium leiognathi
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium damselae
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium leiognathi JT-SHIZ-145
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Photobacterium damselae O66375
-
-
2.4.3.1 Photobacterium leiognathi A8R0T5
-
-
2.4.3.1 Photobacterium leiognathi JT-SHIZ-145 A8R0T5
-
-
2.4.3.1 Photobacterium sp. A8QYL1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 Ni-NTA column chromatography Photobacterium sp.
2.4.3.1 Ni-NTA column chromatography Photobacterium leiognathi
2.4.3.1 Ni-NTA column chromatography Photobacterium damselae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium leiognathi ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium damselae ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium leiognathi ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium damselae ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium sp. ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium leiognathi ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium damselae ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium sp. CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium leiognathi CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium damselae CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium leiognathi JT-SHIZ-145 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha(2,6)-sialyltransferase
-
 Photobacterium sp.
2.4.3.1 alpha(2,6)-sialyltransferase
-
 Photobacterium leiognathi
2.4.3.1 alpha(2,6)-sialyltransferase
-
 Photobacterium damselae
2.4.3.1 alpha(2,6)-ST
-
 Photobacterium sp.
2.4.3.1 alpha(2,6)-ST
-
 Photobacterium leiognathi
2.4.3.1 alpha(2,6)-ST
-
 Photobacterium damselae