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Literature summary extracted from

  • Cornejo, J.; Willows, R.; Beale, S.
    Phytobilin biosynthesis: Cloning and expression of a gene encoding soluble ferredoxin-dependent heme oxygenase from Synechocystis sp. PCC 6803 (1998), Plant J., 15, 99-107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.20 expressed in Escherichia coli BL21(DE3) cells Synechocystis sp. PCC 6803

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.20 Sn-protoporphyrin IX 25% inhibition at 0.02 mM Synechocystis sp. PCC 6803

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.20 31000
-
-
Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.20 mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechocystis sp. PCC 6803
-
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.20 Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.20 partial purification by nickel chelating column chromatography Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.20 mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
Synechocystis sp. PCC 6803 mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.15.20 ? x * 28540, isoform HO2, calculated from amino acid sequence Synechocystis sp. PCC 6803
1.14.15.20 ? x * 31000, isoform HO2, SDS-PAGE Synechocystis sp. PCC 6803

Synonyms

EC Number Synonyms Comment Organism
1.14.15.20 ferredoxin-dependent heme oxygenase
-
Synechocystis sp. PCC 6803
1.14.15.20 Ho1 isoform Synechocystis sp. PCC 6803
1.14.15.20 Ho2 isoform Synechocystis sp. PCC 6803

Expression

EC Number Organism Comment Expression
1.14.15.20 Synechocystis sp. PCC 6803 enzyme expression increases about 7fold during incubation of cells for 72 h in iron-deficient medium up