BRENDA - Enzyme Database show

Enzymic 12-hydroxylation and 12-O-methylation of dihydrochelirubine in dihydromacarpine formation by Thalictrum bulgaricum

Kammerer, L.; De-Eknamkul, W.; Zenk, M.H.; Phytochemistry 36, 1409-1416 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.101
2-Methyl-1,2-di(3-pyridyl)-1-propanone
i.e. methyrapone
Thalictrum bulgaricum
1.14.14.101
5-hydroxy-1,4-naphthoquinone
i.e. juglone
Thalictrum bulgaricum
1.14.14.101
CO
inhibition in the dark, effect is partly reversible upon illumination
Thalictrum bulgaricum
1.14.14.101
cytochrome c
-
Thalictrum bulgaricum
1.14.14.101
ketoconazole
-
Thalictrum bulgaricum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.101
microsome
associated with
Thalictrum bulgaricum
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum bulgaricum
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
Thalictrum bulgaricum
involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.101
Thalictrum bulgaricum
-
-
-
2.1.1.120
Thalictrum bulgaricum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.120
-
Thalictrum bulgaricum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.101
cell culture
yeast-elicited cells
Thalictrum bulgaricum
-
2.1.1.120
cell culture
-
Thalictrum bulgaricum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
-
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7393
Thalictrum bulgaricum
-
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
-
7393
Thalictrum bulgaricum
S-adenosyl-L-homocysteine + dihydromacarpine
-
7393
Thalictrum bulgaricum
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation
7393
Thalictrum bulgaricum
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.101
30
-
-
Thalictrum bulgaricum
2.1.1.120
30
-
-
Thalictrum bulgaricum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.101
8.5
-
-
Thalictrum bulgaricum
2.1.1.120
7.5
-
-
Thalictrum bulgaricum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.101
cytochrome P450
dependent on
Thalictrum bulgaricum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.101
cytochrome P450
dependent on
Thalictrum bulgaricum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.101
2-Methyl-1,2-di(3-pyridyl)-1-propanone
i.e. methyrapone
Thalictrum bulgaricum
1.14.14.101
5-hydroxy-1,4-naphthoquinone
i.e. juglone
Thalictrum bulgaricum
1.14.14.101
CO
inhibition in the dark, effect is partly reversible upon illumination
Thalictrum bulgaricum
1.14.14.101
cytochrome c
-
Thalictrum bulgaricum
1.14.14.101
ketoconazole
-
Thalictrum bulgaricum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.101
microsome
associated with
Thalictrum bulgaricum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum bulgaricum
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
Thalictrum bulgaricum
involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.120
-
Thalictrum bulgaricum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.101
cell culture
yeast-elicited cells
Thalictrum bulgaricum
-
2.1.1.120
cell culture
-
Thalictrum bulgaricum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
-
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7393
Thalictrum bulgaricum
-
1.14.14.101
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
-
7393
Thalictrum bulgaricum
S-adenosyl-L-homocysteine + dihydromacarpine
-
7393
Thalictrum bulgaricum
-
2.1.1.120
S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation
7393
Thalictrum bulgaricum
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.101
30
-
-
Thalictrum bulgaricum
2.1.1.120
30
-
-
Thalictrum bulgaricum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.101
8.5
-
-
Thalictrum bulgaricum
2.1.1.120
7.5
-
-
Thalictrum bulgaricum