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Literature summary extracted from

  • Liu, Y.; Luo, S.H.; Schmidt, A.; Wang, G.D.; Sun, G.L.; Grant, M.; Kuang, C.; Yang, M.J.; Jing, S.X.; Li, C.H.; Schneider, B.; Gershenzon, J.; Li, S.H.
    A geranylfarnesyl diphosphate synthase provides the precursor for sesterterpenoid (C25) formation in the glandular trichomes of the mint species Leucosceptrum canum (2016), Plant Cell, 28, 804-822.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.81 Tween 20 highly activating Leucosceptrum canum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.81 gene Lc-GFDPS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression in Escherichia coli, and in Arabidopsis thaliana driven by a double CaMV 35S promoter Leucosceptrum canum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.81 0.00165
-
 geranylgeranyl diphosphate pH 7.5, 30°C Leucosceptrum canum
2.5.1.81 0.00304
-
 isopentenyl diphosphate pH 7.5, 30°C Leucosceptrum canum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.81 chloroplast
-
 Leucosceptrum canum 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.81 additional information no activation by Mg2+ and Mn2+ Leucosceptrum canum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate Leucosceptrum canum
-
 (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.81 Leucosceptrum canum
-
 gene Lc-GFDPS
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.81 glandular trichome high expression level Leucosceptrum canum
-
2.5.1.81 leaf
-
 Leucosceptrum canum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.81 geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Leucosceptrum canum (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.81 GFDP synthase
-
 Leucosceptrum canum
2.5.1.81 GFDPS
-
 Leucosceptrum canum
2.5.1.81 IDS3
-
 Leucosceptrum canum
2.5.1.81 Lc-GFDPS
-
 Leucosceptrum canum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.81 30
-
 assay at Leucosceptrum canum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.81 0.00000517
-
 geranylgeranyl diphosphate pH 7.5, 30°C Leucosceptrum canum
2.5.1.81 0.0000082
-
 isopentenyl diphosphate pH 7.5, 30°C Leucosceptrum canum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.81 7.5
-
 assay at Leucosceptrum canum

Expression

EC Number Organism Comment Expression
2.5.1.81 Leucosceptrum canum application of salicilic acid and methyl jasmonate induce GFDPS transcript and sesterterpenoid accumulation up

General Information

EC Number General Information Comment Organism
2.5.1.81 evolution phylogenetic analysis suggest that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. Lc-GFDPS clusters in subcluster Ia, which is dominated by GGDPS sequences Leucosceptrum canum
2.5.1.81 metabolism biosynthesis of the C25 precursor geranylfarnesyl diphosphate to leucosceptroid sesterterpenoids is catalyzed by enzyme GFDPS that uses substrates geranylgeranyl diphosphate and Iisopentenyl diphosphate derived from the plastidial MEP pathway, overview Leucosceptrum canum
2.5.1.81 physiological function the geranylfarnesyl diphosphate synthase provides the precursor for sesterterpenoid (C25) formation in the glandular trichomes of the mint species Leucosceptrum canum, the C25 terpenoids might play a defensive role Leucosceptrum canum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.81 0.0028
-
 isopentenyl diphosphate pH 7.5, 30°C Leucosceptrum canum
2.5.1.81 0.003
-
 geranylgeranyl diphosphate pH 7.5, 30°C Leucosceptrum canum