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Literature summary extracted from

  • Chidgey, J.W.; Linhartova, M.; Komenda, J.; Jackson, P.J.; Dickman, M.J.; Canniffe, D.P.; Konik, P.; Pilny, J.; Hunter, C.N.; Sobotka, R.
    A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase (2014), Plant Cell, 26, 1267-1279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.62 gene chlG, recombinant expression of N-terminally 3xFlag-tagged Chl synthase in an CHS-enzyme-deficient Synechocystis sp. strain. The cellular level of FLAG-ChlG is lower than of the native ChlG but apparently sufficient to provide chlorophyll for maintaining normal levels of photosynthetic complexes Synechocystis sp. PCC 6803

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.62 additional information construction of a yidC-Flag/DELTAyidC strain that produces near-native amounts of the C-terminally FLAG-tagged YidC and normal levels of photosynthetic complexes, a very low amount of ChlG coelutes with YidC-FLAG. The HliD-less strain of Synechocystis lacks ChlG subcomplexes and accumulates chlorophyllide. The increased pool of chlorophyllide can be the consequence of lowered ChlG activity but can also arise from a defect in chlorophyll recycling Synechocystis sp. PCC 6803

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.62 chloroplast membrane
-
 Synechocystis sp. PCC 6803 31969
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate Synechocystis sp. PCC 6803
-
 geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate Synechocystis sp. PCC 6803
-
 chlorophyll a + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.62 Synechocystis sp. PCC 6803 Q55145 gene chlG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.62 recombinant 3xFLAG-tagged enzyme from Synechocystis cells, copurification of FLAG-ChlG and YidC-FLAG, by immunaffinity chromatography and ultrafiltration Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate
-
 Synechocystis sp. PCC 6803 geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate
-
 Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.62 ChlG
-
 Synechocystis sp. PCC 6803

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.62 30
-
 assay at Synechocystis sp. PCC 6803

General Information

EC Number General Information Comment Organism
2.5.1.62 metabolism identification of a link between chlorophyll biosynthesis and the Sec/YidC-dependent cotranslational insertion of nascent photosystem polypeptides into membranes. This close physical linkage coordinates the arrival of pigments and nascent apoproteins to produce photosynthetic pigment protein complexes with minimal risk of accumulating phototoxic unbound chlorophylls Synechocystis sp. PCC 6803
2.5.1.62 physiological function the enzymatically active complex of cyanobacterial chlorophyll synthase with the high-light-inducible protein HliD associates with the Ycf39 protein, a putative assembly factor for photosystem II, and the YidC/Alb3 insertase, there is also evidence for the presence of SecY and ribosome subunits in the complex. Protein HliD is apparently required for assembly of FLAG-tagged enzyme ChlG into larger complexes with other proteins such as Ycf39. The ChG-HliD subcomplex contains chlorophyll, chlorophyllide, and carotenoids Synechocystis sp. PCC 6803