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Literature summary extracted from

  • Wu, C.L.; Li, B.Y.; Wu, J.L.; Hui, C.F.
    The activity of carp muscle-specific creatine kinase at low temperature is enhanced by decreased hydrophobicity of residue 268 (2014), Physiol. Biochem. Zool., 87, 507-516.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 G268D site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type Oryctolagus cuniculus
2.7.3.2 G268D site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type Cyprinus carpio
2.7.3.2 G268K site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type Oryctolagus cuniculus
2.7.3.2 G268K site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type Cyprinus carpio
2.7.3.2 G268L site-directed mutagenesis, the mutant does not show altered activity at 10°C and pH 8.0 compared to the wild-type Oryctolagus cuniculus
2.7.3.2 G268L site-directed mutagenesis, the mutant does not show altered activity at 10°C and pH 8.0 compared to the wild-type Cyprinus carpio
2.7.3.2 G268N site-directed mutagenesis, the mutant shows increased activity at 10°C compared to the wild-type Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.3.2 in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant M1-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 Cyprinus carpio
2.7.3.2 in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant RM-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Cyprinus carpio Q9YI16
-
-
2.7.3.2 Oryctolagus cuniculus P00563 gene CKM
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 skeletal muscle
-
 Oryctolagus cuniculus
-
2.7.3.2 skeletal muscle
-
 Cyprinus carpio
-

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 M1-CK
-
 Cyprinus carpio
2.7.3.2 RM-CK
-
 Oryctolagus cuniculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.3.2 additional information
-
 the hydrophobicity of enzyme residue 268 affects the biochemical properties of enzyme M1-CK at low temperatures Cyprinus carpio
2.7.3.2 additional information
-
 the hydrophobicity of enzyme residue 268 affects the biochemical properties of enzyme RM-CK at low temperatures Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 8
-
 assay at Cyprinus carpio