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Literature summary extracted from

  • Lobo-Rojas, T.; Gonzalez-Marcano, E.; Valera-Vera, E.; Acosta, H.; Quinones, W.; Burchmore, R.; Concepcion, J.; Caceres, A.
    Trypanosoma cruzi contains two galactokinases; molecular and biochemical characterization (2016), Parasitol. Int., 65, 472-482.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.6 KCl 50-100 mM, about 80% increase in activity Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 ATP isoform GalK2; isoform GalK2 Trypanosoma cruzi
2.7.1.6 NaCl 50–500 mM, the enzymatic activity decreases approximately 40%–60%, respectively; 50–500 mM, the enzymatic activity decreases approximately 40%–60%, respectively Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.091
-
 D-galactose pH 8.6, temperature not specified in the publication Trypanosoma cruzi
2.7.1.6 0.1
-
 ATP pH 8.6, temperature not specified in the publication Trypanosoma cruzi
2.7.1.6 0.108
-
 D-galactose pH 8.6, temperature not specified in the publication Trypanosoma cruzi
2.7.1.6 0.36
-
 ATP pH 8.6, temperature not specified in the publication Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.6 glycosome both isoforms GalK1 and GalK2 Trypanosoma cruzi 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Mg2+ most effective divalent cation Trypanosoma cruzi
2.7.1.6 Mn2+ 62% of the activity with Mg2+ Trypanosoma cruzi
2.7.1.6 additional information divalent cation required Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 50000
-
 gel filtration, both isoforms GalK1 and GalK2 Trypanosoma cruzi
2.7.1.6 51300
-
-
 Trypanosoma cruzi
2.7.1.6 51900
-
-
 Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Trypanosoma cruzi Q4DF18 isoform Galk1
-
2.7.1.6 Trypanosoma cruzi Q4DFH6 isoform GALK2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.6 epimastigote expression of both isoforms GalK1 and GalK2 Trypanosoma cruzi
-
2.7.1.6 trypomastigote expression of both isoforms GalK1 and GalK2 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + D-galactose
-
 Trypanosoma cruzi ADP + D-galactose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.6 monomer 1 * 51300, calculated, 1 * 50000, SDS-PAGE Trypanosoma cruzi
2.7.1.6 monomer 1 * 51900, calculated, 1 * 50000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 CLB.507001.110
-
 Trypanosoma cruzi
2.7.1.6 CLB.510667.120
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 0.38
-
 D-galactose pH 8.6, temperature not specified in the publication Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 7.5 9.5
-
 Trypanosoma cruzi
2.7.1.6 7.5 9.5 isoform GalK1 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.6 0.41
-
 ATP isoform GalK2, pH 8.6, temperature not specified in the publication Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 3.5
-
 D-galactose pH 8.6, temperature not specified in the publication Trypanosoma cruzi