BRENDA - Enzyme Database

Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1

Topalis, D.; Nogueira, T.C.; De Schutter, T.; El Amri, C.; Krecmerova, M.; Naesens, L.; Balzarini, J.; Andrei, G.; Snoeck, R.; Oncotarget 7, 10386-10401 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
gene CMPK1, genotyping in cervical tumour cell lines
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
additional information
modeling studies of the P64T and R134M amino acid changes, structure-function relationship analysis of mutant UMP/CMPK1s, effect of the two mutations on the stability of the UMP/CMPK1 protein is assessed by circular dichroism, overview
Homo sapiens
2.7.4.14
P64T
naturally occuring mutation, involved in alterations of cidofovir metabolism and resistance against cidofovir in cervical tumour cell lines, thermoresistant mutant compared to the wild-type enzyme. The P64T substitution may modify the distance between Ile62, Val63 and CMP, and therefore reduce the intensity or abolish the interactions observed in the wild-type UMP/CMPK1 at this position
Homo sapiens
2.7.4.14
R134M
naturally occuring mutation, involved in alterations of cidofovir metabolism and resistance against cidofovir in cervical tumour cell lines, thermosensitive similar to the wild-type enzyme. The typical interactions established between an arginine to bridge the phosphate of ATP and CMP for the enzymatic reaction are abolished in the presence of Met134
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.1
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.11
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.123
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.25
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.315
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.327
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.97
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
1.034
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
2.08
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
2.3
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytosol
-
Homo sapiens
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + 5-aza-cidofovir
Homo sapiens
-
ADP + phospho-5-aza-cidofovir
-
-
?
2.7.4.14
ATP + cidofovir
Homo sapiens
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
ADP + phospho-cidofovir
-
-
?
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + cytarabine
Homo sapiens
anticancer drug, phosporylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
ADP + phospho-cytarabine
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
-
ADP + dCDP
-
-
?
2.7.4.14
ATP + dUMP
Homo sapiens
-
ADP + dUDP
-
-
?
2.7.4.14
ATP + gemcitabine
Homo sapiens
i.e. 2',2'-difluorodeoxycytidine
ADP + phospho-gemcitabine
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
2.7.4.14
additional information
Homo sapiens
hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
P30085
gene CMPK1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
HaCaT cell
cidofovir-resistant tumour cell line, no infection with human papilloma virus
Homo sapiens
-
2.7.4.14
HeLa cell
cidofovir-resistant tumour cell line, infection with human papilloma virus
Homo sapiens
-
2.7.4.14
SiHa cell
cidofovir-resistant tumour cell line, infection with human papilloma virus
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 5-aza-cidofovir
-
739215
Homo sapiens
ADP + phospho-5-aza-cidofovir
-
-
-
?
2.7.4.14
ATP + 5-aza-cidofovir
wild-type enzyme
739215
Homo sapiens
ADP + phospho-5-aza-cidofovir
-
-
-
?
2.7.4.14
ATP + cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
739215
Homo sapiens
ADP + phospho-cidofovir
-
-
-
?
2.7.4.14
ATP + cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid, wild-type enzyme
739215
Homo sapiens
ADP + phospho-cidofovir
-
-
-
?
2.7.4.14
ATP + CMP
-
739215
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + cytarabine
anticancer drug, phosporylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
739215
Homo sapiens
ADP + phospho-cytarabine
-
-
-
?
2.7.4.14
ATP + cytarabine
i.e. araC, wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + phospho-cytarabine
-
-
-
?
2.7.4.14
ATP + dCMP
-
739215
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dCMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
739215
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + dUMP
wild-type enzyme
739215
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + gemcitabine
i.e. 2',2'-difluorodeoxycytidine
739215
Homo sapiens
ADP + phospho-gemcitabine
-
-
-
?
2.7.4.14
ATP + gemcitabine
i.e. 2',2'-difluorodeoxycytidine, wild-type enzyme
739215
Homo sapiens
ADP + phospho-gemcitabine
-
-
-
?
2.7.4.14
ATP + UMP
-
739215
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
739215
Homo sapiens
?
-
-
-
-
2.7.4.14
additional information
mutants P64T/R134M and R134M show no activity with all substrates
739215
Homo sapiens
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.14
70
-
inactivation of wild-type enzyme and mutant R134M enzyme, only partial denaturation of mutant P64T
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.019
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.036
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.09
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.31
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.35
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.47
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
5.8
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
13.4
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
125
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
130
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
188
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
gene CMPK1, genotyping in cervical tumour cell lines
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
additional information
modeling studies of the P64T and R134M amino acid changes, structure-function relationship analysis of mutant UMP/CMPK1s, effect of the two mutations on the stability of the UMP/CMPK1 protein is assessed by circular dichroism, overview
Homo sapiens
2.7.4.14
P64T
naturally occuring mutation, involved in alterations of cidofovir metabolism and resistance against cidofovir in cervical tumour cell lines, thermoresistant mutant compared to the wild-type enzyme. The P64T substitution may modify the distance between Ile62, Val63 and CMP, and therefore reduce the intensity or abolish the interactions observed in the wild-type UMP/CMPK1 at this position
Homo sapiens
2.7.4.14
R134M
naturally occuring mutation, involved in alterations of cidofovir metabolism and resistance against cidofovir in cervical tumour cell lines, thermosensitive similar to the wild-type enzyme. The typical interactions established between an arginine to bridge the phosphate of ATP and CMP for the enzymatic reaction are abolished in the presence of Met134
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.1
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.11
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.123
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.25
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.315
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.327
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.97
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
1.034
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
2.08
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
2.3
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytosol
-
Homo sapiens
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + 5-aza-cidofovir
Homo sapiens
-
ADP + phospho-5-aza-cidofovir
-
-
?
2.7.4.14
ATP + cidofovir
Homo sapiens
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
ADP + phospho-cidofovir
-
-
?
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + cytarabine
Homo sapiens
anticancer drug, phosporylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
ADP + phospho-cytarabine
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
-
ADP + dCDP
-
-
?
2.7.4.14
ATP + dUMP
Homo sapiens
-
ADP + dUDP
-
-
?
2.7.4.14
ATP + gemcitabine
Homo sapiens
i.e. 2',2'-difluorodeoxycytidine
ADP + phospho-gemcitabine
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
2.7.4.14
additional information
Homo sapiens
hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
HaCaT cell
cidofovir-resistant tumour cell line, no infection with human papilloma virus
Homo sapiens
-
2.7.4.14
HeLa cell
cidofovir-resistant tumour cell line, infection with human papilloma virus
Homo sapiens
-
2.7.4.14
SiHa cell
cidofovir-resistant tumour cell line, infection with human papilloma virus
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 5-aza-cidofovir
-
739215
Homo sapiens
ADP + phospho-5-aza-cidofovir
-
-
-
?
2.7.4.14
ATP + 5-aza-cidofovir
wild-type enzyme
739215
Homo sapiens
ADP + phospho-5-aza-cidofovir
-
-
-
?
2.7.4.14
ATP + cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
739215
Homo sapiens
ADP + phospho-cidofovir
-
-
-
?
2.7.4.14
ATP + cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid, wild-type enzyme
739215
Homo sapiens
ADP + phospho-cidofovir
-
-
-
?
2.7.4.14
ATP + CMP
-
739215
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + cytarabine
anticancer drug, phosporylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
739215
Homo sapiens
ADP + phospho-cytarabine
-
-
-
?
2.7.4.14
ATP + cytarabine
i.e. araC, wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + phospho-cytarabine
-
-
-
?
2.7.4.14
ATP + dCMP
-
739215
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dCMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
739215
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + dUMP
wild-type enzyme
739215
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + gemcitabine
i.e. 2',2'-difluorodeoxycytidine
739215
Homo sapiens
ADP + phospho-gemcitabine
-
-
-
?
2.7.4.14
ATP + gemcitabine
i.e. 2',2'-difluorodeoxycytidine, wild-type enzyme
739215
Homo sapiens
ADP + phospho-gemcitabine
-
-
-
?
2.7.4.14
ATP + UMP
-
739215
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
wild-type enzyme and mutant P64T
739215
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
739215
Homo sapiens
?
-
-
-
-
2.7.4.14
additional information
mutants P64T/R134M and R134M show no activity with all substrates
739215
Homo sapiens
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.14
70
-
inactivation of wild-type enzyme and mutant R134M enzyme, only partial denaturation of mutant P64T
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.019
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.036
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.09
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.31
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.35
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.47
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
5.8
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
13.4
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
125
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
130
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
188
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.7.4.14
malfunction
mutations P64T and R134M as well as downregulation of UMP/CMPK1 expression are observed in SiHaCDV and HeLaCDV, respectively, leading to alterations of cidofovir metabolism and resistance against cidofovir in the cervical tumour cell lines. In HaCaTCDV, no mutations of the uck gene are found and no impairment of UMP/CMPK1 activity is observed
Homo sapiens
2.7.4.14
metabolism
the first and last phosphorylation steps of cytidine 2'-deoxynucleoside analogues are catalyzed, respectively, by deoxycytidine kinase (dCK) and nucleoside diphosphate kinase (NDPK)
Homo sapiens
2.7.4.14
additional information
anticancer drugs, analogues of 2'-deoxycytidine (dC) (i.e. lamivudine and emtricitabine) or 2'-deoxycytidine monophosphate (dCMP) (i.e. CDV), require phosphorylation by UMP/CMPK. The anticancer agents cytarabine (araC) and gemcitabine (dFdC) are activated by UMP/CMPK1 following prior conversion to their 5?-monophosphate forms by dCK
Homo sapiens
2.7.4.14
physiological function
the cytosolic UMP/CMP kinase 1, is a key enzyme in the activation of antiviral and anticancer drugs and catalyzes the phosphorylation of (d)CMP analogues to their diphosphate forms
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.4.14
malfunction
mutations P64T and R134M as well as downregulation of UMP/CMPK1 expression are observed in SiHaCDV and HeLaCDV, respectively, leading to alterations of cidofovir metabolism and resistance against cidofovir in the cervical tumour cell lines. In HaCaTCDV, no mutations of the uck gene are found and no impairment of UMP/CMPK1 activity is observed
Homo sapiens
2.7.4.14
metabolism
the first and last phosphorylation steps of cytidine 2'-deoxynucleoside analogues are catalyzed, respectively, by deoxycytidine kinase (dCK) and nucleoside diphosphate kinase (NDPK)
Homo sapiens
2.7.4.14
additional information
anticancer drugs, analogues of 2'-deoxycytidine (dC) (i.e. lamivudine and emtricitabine) or 2'-deoxycytidine monophosphate (dCMP) (i.e. CDV), require phosphorylation by UMP/CMPK. The anticancer agents cytarabine (araC) and gemcitabine (dFdC) are activated by UMP/CMPK1 following prior conversion to their 5?-monophosphate forms by dCK
Homo sapiens
2.7.4.14
physiological function
the cytosolic UMP/CMP kinase 1, is a key enzyme in the activation of antiviral and anticancer drugs and catalyzes the phosphorylation of (d)CMP analogues to their diphosphate forms
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.037
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.04
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.058
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.226
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
1.4
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
2.52
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
5.61
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
122
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
410
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
1880
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
6250
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.037
-
dCMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.04
-
Cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
0.058
-
cytarabine
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
0.226
-
5-aza-cidofovir
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
1.4
-
UMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
2.52
-
CMP
pH and temperature not specified in the publication, recombinant mutant P64T
Homo sapiens
2.7.4.14
5.61
-
dUMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
122
-
dCMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
410
-
cytarabine
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
1880
-
UMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens
2.7.4.14
6250
-
CMP
pH and temperature not specified in the publication, recombinant wild-type enzyme
Homo sapiens