BRENDA - Enzyme Database show

Enzymic 10-hydroxylation and 10-O-methylation of dihydrosanguinarine in dihydrochelirubine formation by Eschscholtzia

De-Eknamkul, W.; Tanahashi, T.; Zenk, M.H.; Phytochemistry 31, 2713-2717 (1992)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.100
Ancymidol
-
Eschscholzia californica
1.14.14.100
CO
strong inhibition in the dark, effect is partly reversible by illumination
Eschscholzia californica
1.14.14.100
cytochrome c
-
Eschscholzia californica
1.14.14.100
ketoconazole
-
Eschscholzia californica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.119
0.003
-
S-adenosyl-L-methionine
-
Eschscholzia californica
2.1.1.119
0.01
-
10-Hydroxydihydrosanguinarine
-
Eschscholzia californica
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.100
microsome
associated with
Eschscholzia californica
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.119
49000
-
gel filtration
Eschscholzia californica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
Eschscholzia californica
the enzyme is involved in benzophenanthridine alkaloid biosynthesis
S-adenosyl-L-homocysteine + dihydrochelirubine
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.100
Eschscholzia californica
-
-
-
2.1.1.119
Eschscholzia californica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.119
-
Eschscholzia californica
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.100
cell suspension culture
-
Eschscholzia californica
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.119
additional information
-
-
Eschscholzia californica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.100
dihydrosanguinarine + [reduced NADPH-hemoprotein reductase] + O2
-
7392
Eschscholzia californica
10-hydroxydihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7392
Eschscholzia californica
-
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
high substrate specificity
7392
Eschscholzia californica
S-adenosyl-L-homocysteine + dihydrochelirubine
-
7392
Eschscholzia californica
-
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
the enzyme is involved in benzophenanthridine alkaloid biosynthesis
7392
Eschscholzia californica
S-adenosyl-L-homocysteine + dihydrochelirubine
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.119
30
40
-
Eschscholzia californica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.119
8.5
-
-
Eschscholzia californica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.100
cytochrome P450
dependent on
Eschscholzia californica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.100
cytochrome P450
dependent on
Eschscholzia californica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.100
Ancymidol
-
Eschscholzia californica
1.14.14.100
CO
strong inhibition in the dark, effect is partly reversible by illumination
Eschscholzia californica
1.14.14.100
cytochrome c
-
Eschscholzia californica
1.14.14.100
ketoconazole
-
Eschscholzia californica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.119
0.003
-
S-adenosyl-L-methionine
-
Eschscholzia californica
2.1.1.119
0.01
-
10-Hydroxydihydrosanguinarine
-
Eschscholzia californica
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.100
microsome
associated with
Eschscholzia californica
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.119
49000
-
gel filtration
Eschscholzia californica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
Eschscholzia californica
the enzyme is involved in benzophenanthridine alkaloid biosynthesis
S-adenosyl-L-homocysteine + dihydrochelirubine
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.119
-
Eschscholzia californica
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.100
cell suspension culture
-
Eschscholzia californica
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.119
additional information
-
-
Eschscholzia californica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.100
dihydrosanguinarine + [reduced NADPH-hemoprotein reductase] + O2
-
7392
Eschscholzia californica
10-hydroxydihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7392
Eschscholzia californica
-
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
high substrate specificity
7392
Eschscholzia californica
S-adenosyl-L-homocysteine + dihydrochelirubine
-
7392
Eschscholzia californica
-
2.1.1.119
S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine
the enzyme is involved in benzophenanthridine alkaloid biosynthesis
7392
Eschscholzia californica
S-adenosyl-L-homocysteine + dihydrochelirubine
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.119
30
40
-
Eschscholzia californica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.119
8.5
-
-
Eschscholzia californica