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Literature summary extracted from

  • De-Eknamkul, W.; Tanahashi, T.; Zenk, M.H.
    Enzymic 10-hydroxylation and 10-O-methylation of dihydrosanguinarine in dihydrochelirubine formation by Eschscholtzia (1992), Phytochemistry, 31, 2713-2717.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.100 Ancymidol
-
 Eschscholzia californica
1.14.14.100 CO strong inhibition in the dark, effect is partly reversible by illumination Eschscholzia californica
1.14.14.100 cytochrome c
-
 Eschscholzia californica
1.14.14.100 ketoconazole
-
 Eschscholzia californica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.119 0.003
-
 S-adenosyl-L-methionine
-
 Eschscholzia californica
2.1.1.119 0.01
-
 10-Hydroxydihydrosanguinarine
-
 Eschscholzia californica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.100 microsome associated with Eschscholzia californica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.119 49000
-
 gel filtration Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.119 S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine Eschscholzia californica the enzyme is involved in benzophenanthridine alkaloid biosynthesis S-adenosyl-L-homocysteine + dihydrochelirubine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.100 Eschscholzia californica
-
-
-
2.1.1.119 Eschscholzia californica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.119
-
 Eschscholzia californica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.100 cell suspension culture
-
 Eschscholzia californica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.119 additional information
-
-
 Eschscholzia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.100 dihydrosanguinarine + [reduced NADPH-hemoprotein reductase] + O2
-
 Eschscholzia californica 10-hydroxydihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.1.1.119 S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine high substrate specificity Eschscholzia californica S-adenosyl-L-homocysteine + dihydrochelirubine
-
 ?
2.1.1.119 S-adenosyl-L-methionine + 10-hydroxydihydrosanguinarine the enzyme is involved in benzophenanthridine alkaloid biosynthesis Eschscholzia californica S-adenosyl-L-homocysteine + dihydrochelirubine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.119 30 40
-
 Eschscholzia californica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.119 8.5
-
-
 Eschscholzia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.100 cytochrome P450 dependent on Eschscholzia californica