EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.48 | gene rrpC, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis | Dictyostelium discoideum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.48 | additional information | rrpC gene knockout by gene disruption | Dictyostelium discoideum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | Dictyostelium discoideum | - |
diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | Dictyostelium discoideum AX2 | - |
diphosphate + RNAn+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.48 | Dictyostelium discoideum | Q54UI6 | gene rrpC | - |
2.7.7.48 | Dictyostelium discoideum AX2 | Q54UI6 | gene rrpC | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | - |
Dictyostelium discoideum | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | - |
Dictyostelium discoideum AX2 | diphosphate + RNAn+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.48 | RNA-dependent RNA polymerase | - |
Dictyostelium discoideum |
2.7.7.48 | RrpC | - |
Dictyostelium discoideum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.48 | malfunction | in deletion strains of the RNA-dependent RNA polymerase RrpC, full-length and shorter retrotransposable element DIRS-1 (Dictyostelium intermediate repeat sequence 1) messenger RNAs are strongly enriched, shorter versions of long non-coding RNA in DIRS-1 antisense orientation are also enriched in rrpC- strains. DIRS-1 misregulation in the absence of RrpC leads to retrotransposon mobilization | Dictyostelium discoideum |
2.7.7.48 | physiological function | the RNA-dependent RNA polymerase RrpC silences the centromeric retrotransposon DIRS-1 post-transcriptionally and is required for the spreading of RNA silencing signals. RrpC is a key player in the silencing of centromeric retrotransposon DIRS-1. RrpC acts at the post-transcriptional level and is involved in spreading of RNA silencing signals, both in the 5' and 3' directions | Dictyostelium discoideum |