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Literature summary extracted from

  • Yu, M.; Huang, M.; Song, Q.; Shao, J.; Ying, X.
    Characterization of a (2R,3R)-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (2015), Molecules, 20, 7156-7173.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.4 DMSO the addition of 10% (v/v) dimethyl sulfoxide increases the activity by 161.2% Rhodococcus erythropolis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 expressed in Escherichia coli BL21(DE3) cells Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.4 acetone 38.3% residual activity at 10% (v/v) Rhodococcus erythropolis
1.1.1.4 acetonitrile 2.4% residual activity at 10% (v/v) Rhodococcus erythropolis
1.1.1.4 Ag+ complete inhibition at 1 mM Rhodococcus erythropolis
1.1.1.4 Al3+ 21.6% residual activity at 1 mM Rhodococcus erythropolis
1.1.1.4 Ba2+ 85.8% residual activity at 1 mM Rhodococcus erythropolis
1.1.1.4 Ca2+ 89.3% residual activity at 1 mM Rhodococcus erythropolis
1.1.1.4 Co2+ 79.5% residual activity at 1 mM Rhodococcus erythropolis
1.1.1.4 Cu2+ complete inhibition at 1 mM Rhodococcus erythropolis
1.1.1.4 DMSO 8.4% residual activity at 40% (v/v) Rhodococcus erythropolis
1.1.1.4 EDTA
-
Rhodococcus erythropolis
1.1.1.4 ethanol 3.7% residual activity at 10% (v/v) Rhodococcus erythropolis
1.1.1.4 Fe2+ complete inhibition at 1 mM Rhodococcus erythropolis
1.1.1.4 methanol 14% residual activity at 10% (v/v) Rhodococcus erythropolis
1.1.1.4 Mn2+ 81.1% residual activity at 1 mM Rhodococcus erythropolis
1.1.1.4 Zn2+ 3.4% residual activity at 1 mM Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.08
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45°C Rhodococcus erythropolis
1.1.1.4 0.1
-
diacetyl at pH 10.0 and 45°C Rhodococcus erythropolis
1.1.1.4 0.58
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55°C Rhodococcus erythropolis
1.1.1.4 1.04
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.4 K+ 138.9% activity at 1 mM Rhodococcus erythropolis
1.1.1.4 additional information Na+ and Mg2+ cause no significant activation Rhodococcus erythropolis
1.1.1.4 Zn2+ a zinc-containing alcohol dehydrogenase Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 44000
-
-
Rhodococcus erythropolis
1.1.1.4 45000
-
gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Rhodococcus erythropolis
-
(R)-acetoin + NADH + H+
-
r
1.1.1.4 (R)-acetoin + NADH + H+ Rhodococcus erythropolis the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 (2R,3R)-butane-2,3-diol + NAD+
-
r
1.1.1.4 (R)-acetoin + NADH + H+ Rhodococcus erythropolis WZ010 the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 (2R,3R)-butane-2,3-diol + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Rhodococcus erythropolis A0A0E4A9D6
-
-
1.1.1.4 Rhodococcus erythropolis WZ010 A0A0E4A9D6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
Rhodococcus erythropolis (R)-acetoin + NADH + H+
-
r
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ 69.5% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
1.1.1.4 (R)-1-phenyl-1,2-ethanediol + NAD+ 32.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
1.1.1.4 (R)-acetoin + NADH + H+ the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 Rhodococcus erythropolis (2R,3R)-butane-2,3-diol + NAD+
-
r
1.1.1.4 (R)-acetoin + NADH + H+ the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 Rhodococcus erythropolis WZ010 (2R,3R)-butane-2,3-diol + NAD+
-
r
1.1.1.4 2,2,2-trifluoroacetophenone + NADH + H+ 28% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
1.1.1.4 2,2,2-trifluoroacetophenone + NADH + H+ 28% activity compared to (R)-acetoin Rhodococcus erythropolis WZ010 ?
-
?
1.1.1.4 2-hydroxyacetophenone + NADH + H+ 8.7% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
1.1.1.4 2-octanone + NADH + H+ 9.3% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
1.1.1.4 3-methyl-2-butenal + NADH + H+ 16.7% activity compared to (R)-acetoin Rhodococcus erythropolis ?
-
?
1.1.1.4 butane-1,3-diol + NAD+ 14.7% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
1.1.1.4 diacetyl + NADH + H+ 69.3% activity compared to (R)-acetoin Rhodococcus erythropolis (R)-acetoin + NAD+
-
r
1.1.1.4 diacetyl + NADH + H+ 69.3% activity compared to (R)-acetoin Rhodococcus erythropolis WZ010 (R)-acetoin + NAD+
-
r
1.1.1.4 ethyl 4-chloro-3-hydroxybutyrate + NAD+ 12.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
1.1.1.4 glycerol + NAD+ 16.3% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?
1.1.1.4 glycerol + NAD+ 16.3% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis WZ010 ?
-
?
1.1.1.4 additional information no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone Rhodococcus erythropolis ?
-
?
1.1.1.4 additional information no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone Rhodococcus erythropolis WZ010 ?
-
?
1.1.1.4 sodium lactate + NAD+ 10.2% activity compared to (2R,3R)-butane-2,3-diol Rhodococcus erythropolis ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.4 monomer 1 * 37180, calculated from amino acid sequence Rhodococcus erythropolis
1.1.1.4 monomer 1 * 44000, His-tagged enzyme, SDS-PAGE Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 (2R,3R)-2,3-butanediol dehydrogenase
-
Rhodococcus erythropolis
1.1.1.4 BDH
-
Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.4 45
-
for (2R,3R)-butane-2,3-diol oxidation Rhodococcus erythropolis
1.1.1.4 55
-
for acetoin reduction Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.4 45
-
after 6 h incubation at 45°C, the remaining activities for acetoin reduction and butane-2,3-diol oxidation are 49.7% and 43.5%, respectively Rhodococcus erythropolis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 4.28
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55°C Rhodococcus erythropolis
1.1.1.4 5.73
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45°C Rhodococcus erythropolis
1.1.1.4 6.11
-
diacetyl at pH 10.0 and 45°C Rhodococcus erythropolis
1.1.1.4 10.27
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 6.5
-
for acetoin reduction Rhodococcus erythropolis
1.1.1.4 10
-
for (2R,3R)-butane-2,3-diol oxidation Rhodococcus erythropolis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.4 6.5 7 for the reduction of acetoin, more than 85% of its maximal activity is in the pH range of 6.5 to 7.0 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
Rhodococcus erythropolis
1.1.1.4 NADH
-
Rhodococcus erythropolis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 7.379
-
(2R,3R)-butane-2,3-diol at pH 6.5 and 55°C Rhodococcus erythropolis
1.1.1.4 9.875
-
NAD+ with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C Rhodococcus erythropolis
1.1.1.4 61.1
-
diacetyl at pH 10.0 and 45°C Rhodococcus erythropolis
1.1.1.4 71.63
-
NADH with diacetyl as cosubstrate, at pH 10.0 and 45°C Rhodococcus erythropolis