EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | DMSO | the addition of 10% (v/v) dimethyl sulfoxide increases the activity by 161.2% | Rhodococcus erythropolis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | expressed in Escherichia coli BL21(DE3) cells | Rhodococcus erythropolis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | acetone | 38.3% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
1.1.1.4 | acetonitrile | 2.4% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
1.1.1.4 | Ag+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Al3+ | 21.6% residual activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Ba2+ | 85.8% residual activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Ca2+ | 89.3% residual activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Co2+ | 79.5% residual activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Cu2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | DMSO | 8.4% residual activity at 40% (v/v) | Rhodococcus erythropolis | |
1.1.1.4 | EDTA | - |
Rhodococcus erythropolis | |
1.1.1.4 | ethanol | 3.7% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
1.1.1.4 | Fe2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | methanol | 14% residual activity at 10% (v/v) | Rhodococcus erythropolis | |
1.1.1.4 | Mn2+ | 81.1% residual activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | Zn2+ | 3.4% residual activity at 1 mM | Rhodococcus erythropolis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.08 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis | |
1.1.1.4 | 0.1 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
1.1.1.4 | 0.58 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
1.1.1.4 | 1.04 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | K+ | 138.9% activity at 1 mM | Rhodococcus erythropolis | |
1.1.1.4 | additional information | Na+ and Mg2+ cause no significant activation | Rhodococcus erythropolis | |
1.1.1.4 | Zn2+ | a zinc-containing alcohol dehydrogenase | Rhodococcus erythropolis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 44000 | - |
- |
Rhodococcus erythropolis |
1.1.1.4 | 45000 | - |
gel filtration | Rhodococcus erythropolis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | Rhodococcus erythropolis | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R)-acetoin + NADH + H+ | Rhodococcus erythropolis | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
1.1.1.4 | (R)-acetoin + NADH + H+ | Rhodococcus erythropolis WZ010 | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Rhodococcus erythropolis | A0A0E4A9D6 | - |
- |
1.1.1.4 | Rhodococcus erythropolis WZ010 | A0A0E4A9D6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.4 | DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography | Rhodococcus erythropolis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Rhodococcus erythropolis | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | 69.5% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | (R)-1-phenyl-1,2-ethanediol + NAD+ | 32.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | (R)-acetoin + NADH + H+ | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | Rhodococcus erythropolis | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
1.1.1.4 | (R)-acetoin + NADH + H+ | the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0 | Rhodococcus erythropolis WZ010 | (2R,3R)-butane-2,3-diol + NAD+ | - |
r | |
1.1.1.4 | 2,2,2-trifluoroacetophenone + NADH + H+ | 28% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | 2,2,2-trifluoroacetophenone + NADH + H+ | 28% activity compared to (R)-acetoin | Rhodococcus erythropolis WZ010 | ? | - |
? | |
1.1.1.4 | 2-hydroxyacetophenone + NADH + H+ | 8.7% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | 2-octanone + NADH + H+ | 9.3% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | 3-methyl-2-butenal + NADH + H+ | 16.7% activity compared to (R)-acetoin | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | butane-1,3-diol + NAD+ | 14.7% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | diacetyl + NADH + H+ | 69.3% activity compared to (R)-acetoin | Rhodococcus erythropolis | (R)-acetoin + NAD+ | - |
r | |
1.1.1.4 | diacetyl + NADH + H+ | 69.3% activity compared to (R)-acetoin | Rhodococcus erythropolis WZ010 | (R)-acetoin + NAD+ | - |
r | |
1.1.1.4 | ethyl 4-chloro-3-hydroxybutyrate + NAD+ | 12.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | glycerol + NAD+ | 16.3% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | glycerol + NAD+ | 16.3% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis WZ010 | ? | - |
? | |
1.1.1.4 | additional information | no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone | Rhodococcus erythropolis | ? | - |
? | |
1.1.1.4 | additional information | no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone | Rhodococcus erythropolis WZ010 | ? | - |
? | |
1.1.1.4 | sodium lactate + NAD+ | 10.2% activity compared to (2R,3R)-butane-2,3-diol | Rhodococcus erythropolis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.4 | monomer | 1 * 37180, calculated from amino acid sequence | Rhodococcus erythropolis |
1.1.1.4 | monomer | 1 * 44000, His-tagged enzyme, SDS-PAGE | Rhodococcus erythropolis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Rhodococcus erythropolis |
1.1.1.4 | BDH | - |
Rhodococcus erythropolis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 45 | - |
for (2R,3R)-butane-2,3-diol oxidation | Rhodococcus erythropolis |
1.1.1.4 | 55 | - |
for acetoin reduction | Rhodococcus erythropolis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 45 | - |
after 6 h incubation at 45°C, the remaining activities for acetoin reduction and butane-2,3-diol oxidation are 49.7% and 43.5%, respectively | Rhodococcus erythropolis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 4.28 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
1.1.1.4 | 5.73 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis | |
1.1.1.4 | 6.11 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
1.1.1.4 | 10.27 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 6.5 | - |
for acetoin reduction | Rhodococcus erythropolis |
1.1.1.4 | 10 | - |
for (2R,3R)-butane-2,3-diol oxidation | Rhodococcus erythropolis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 6.5 | 7 | for the reduction of acetoin, more than 85% of its maximal activity is in the pH range of 6.5 to 7.0 | Rhodococcus erythropolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NAD+ | - |
Rhodococcus erythropolis | |
1.1.1.4 | NADH | - |
Rhodococcus erythropolis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 7.379 | - |
(2R,3R)-butane-2,3-diol | at pH 6.5 and 55°C | Rhodococcus erythropolis | |
1.1.1.4 | 9.875 | - |
NAD+ | with (2R,3R)-butane-2,3-diol as cosubstrate, at pH 6.5 and 55°C | Rhodococcus erythropolis | |
1.1.1.4 | 61.1 | - |
diacetyl | at pH 10.0 and 45°C | Rhodococcus erythropolis | |
1.1.1.4 | 71.63 | - |
NADH | with diacetyl as cosubstrate, at pH 10.0 and 45°C | Rhodococcus erythropolis |