BRENDA - Enzyme Database show

Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis

Tanahashi, T.; Zenk, M.H.; Phytochemistry 29, 1113-1122 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.98
CO
-
Eschscholzia californica
1.14.14.98
cytochrome c
-
Eschscholzia californica
1.14.14.98
ketoconazole
-
Eschscholzia californica
1.14.14.98
Metyrapone
-
Eschscholzia californica
1.14.14.98
Prochloraz
-
Eschscholzia californica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.98
0.002
-
protopine
-
Eschscholzia californica
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.98
microsome
-
Chelidonium majus
-
-
1.14.14.98
microsome
-
Dicentra cucullaria
-
-
1.14.14.98
microsome
membrane-bound
Eschscholzia californica
-
-
1.14.14.98
microsome
-
Eschscholzia lobbii
-
-
1.14.14.98
microsome
-
Fumaria parviflora
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.98
additional information
Eschscholzia californica
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Eschscholzia lobbii
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Chelidonium majus
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Fumaria parviflora
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Dicentra cucullaria
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.98
Chelidonium majus
-
-
-
1.14.14.98
Dicentra cucullaria
-
-
-
1.14.14.98
Eschscholzia californica
-
strain AST, strain BB, strain ROT
-
1.14.14.98
Eschscholzia lobbii
-
-
-
1.14.14.98
Fumaria parviflora
-
-
-
1.14.14.98
no activity in Berberis stolonifera
-
elicited and non-elicited cells
-
1.14.14.98
no activity in Catharanthus roseus
-
elicited and non-elicited cells
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Chelidonium majus
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Dicentra cucullaria
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia californica
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia lobbii
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Fumaria parviflora
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia californica
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia lobbii
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Chelidonium majus
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Fumaria parviflora
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Dicentra cucullaria
?
-
-
-
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia californica
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia californica
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia lobbii
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia lobbii
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Chelidonium majus
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Chelidonium majus
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Fumaria parviflora
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Fumaria parviflora
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Dicentra cucullaria
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Dicentra cucullaria
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.98
30
-
-
Eschscholzia californica
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.14.98
10
40
about 50% of maximal actvity at 10°C and 40°C
Eschscholzia californica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.98
7.5
-
-
Eschscholzia californica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.14.98
6.3
8.5
pH 6.3: about 35% of maximal activity, pH 8.5: about 40% of maximal activity
Eschscholzia californica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.98
cytochrome P450
the enzyme is linked to cytochrome P-450
Eschscholzia californica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.98
cytochrome P450
the enzyme is linked to cytochrome P-450
Eschscholzia californica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.98
CO
-
Eschscholzia californica
1.14.14.98
cytochrome c
-
Eschscholzia californica
1.14.14.98
ketoconazole
-
Eschscholzia californica
1.14.14.98
Metyrapone
-
Eschscholzia californica
1.14.14.98
Prochloraz
-
Eschscholzia californica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.98
0.002
-
protopine
-
Eschscholzia californica
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.98
microsome
-
Chelidonium majus
-
-
1.14.14.98
microsome
-
Dicentra cucullaria
-
-
1.14.14.98
microsome
membrane-bound
Eschscholzia californica
-
-
1.14.14.98
microsome
-
Eschscholzia lobbii
-
-
1.14.14.98
microsome
-
Fumaria parviflora
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.98
additional information
Eschscholzia californica
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Eschscholzia lobbii
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Chelidonium majus
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Fumaria parviflora
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
1.14.14.98
additional information
Dicentra cucullaria
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Chelidonium majus
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Dicentra cucullaria
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia californica
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia lobbii
-
1.14.14.98
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Fumaria parviflora
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia californica
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia lobbii
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Chelidonium majus
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Fumaria parviflora
?
-
-
-
-
1.14.14.98
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Dicentra cucullaria
?
-
-
-
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia californica
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia californica
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia lobbii
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia lobbii
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Chelidonium majus
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Chelidonium majus
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Fumaria parviflora
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Fumaria parviflora
-
1.14.14.98
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Dicentra cucullaria
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Dicentra cucullaria
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.98
30
-
-
Eschscholzia californica
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.14.98
10
40
about 50% of maximal actvity at 10°C and 40°C
Eschscholzia californica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.98
7.5
-
-
Eschscholzia californica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.14.98
6.3
8.5
pH 6.3: about 35% of maximal activity, pH 8.5: about 40% of maximal activity
Eschscholzia californica