EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.21 | biotechnology | ALDRXV4 gene from Xerophyta viscosa is a potential candidate for developing stress-tolerant crop plants | Xerophyta viscosa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.21 | gene ALDRXV4, cDNA expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) using pET28a expression vector. Overexpression of enzyme ALDRXV4 improves growth and tolerance against diverse abiotic stresses induced by high salt (500 mM NaCl), osmoticum (10% PEG 6000), heavy metal (20 mM CdCl2), and methylglyoxal (5 mM) in Escherichia coli cells. Recombinant expression of ALDRXV4-GFP fusion enzyme in onion epidermal peel via Agrobacterium tumefaciens transfection method | Xerophyta viscosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.21 | additional information | - |
additional information | Michaelis-Menten kinetics | Xerophyta viscosa | |
1.1.1.21 | 1.1 | - |
DL-glyceraldehyde | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa | |
1.1.1.21 | 1.2 | - |
methylglyoxal | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.21 | cytoplasm | - |
Xerophyta viscosa | 5737 | - |
1.1.1.21 | nucleus | - |
Xerophyta viscosa | 5634 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.21 | 36000 | - |
- |
Xerophyta viscosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.21 | Xerophyta viscosa | Q9XF81 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.21 | recombinant His-tagged enzyme several fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Xerophyta viscosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.21 | DL-glyceraldehyde + NADPH + H+ | - |
Xerophyta viscosa | glycerol + NADP+ | - |
? | |
1.1.1.21 | malondialdehyde + NADPH + H+ | - |
Xerophyta viscosa | ? | - |
? | |
1.1.1.21 | methylglyoxal + NADPH + H+ | - |
Xerophyta viscosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.21 | ? | x * 36000, about, recombinant enzyme, SDS-PAGE | Xerophyta viscosa |
1.1.1.21 | More | three-dimensional structure prediction based on homology modeling using the the structure of the Hordeum vulgare enzyme, PDB ID 2bgs, and circular dichroism spectroscopy structure analysis, overview | Xerophyta viscosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.21 | aldose reductase | - |
Xerophyta viscosa |
1.1.1.21 | ALDRXV4 | - |
Xerophyta viscosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.21 | 5.8 | - |
DL-glyceraldehyde | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa | |
1.1.1.21 | 14.5 | - |
methylglyoxal | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.21 | 6.2 | - |
assay at | Xerophyta viscosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.21 | NADPH | - |
Xerophyta viscosa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.21 | physiological function | aldose reductases are key enzymes in the detoxification of reactive aldehyde compounds like methylglyoxal and malondialdehyde | Xerophyta viscosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.21 | 5.3 | - |
DL-glyceraldehyde | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa | |
1.1.1.21 | 9.3 | - |
methylglyoxal | pH 6.2, temperature not specified in the publication, recombinant His-tagged enzyme | Xerophyta viscosa |