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Literature summary extracted from

  • Cardello, L.; Terenzi, H. F.; Jorge, J. A.
    A cytosolic trehalase from the thermophilic fungus Humicola grisea var. thermoidea (1994), Microbiology, 140, 1671-1677.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ADP 10 mM, 51% inhibition Trichocladium griseum
3.2.1.28 AlCl3 1 mM, complete inhibition Trichocladium griseum
3.2.1.28 AMP 10 mM, 5% inhibition Trichocladium griseum
3.2.1.28 ATP 10 mM, 67% inhibition Trichocladium griseum
3.2.1.28 cellobiose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
3.2.1.28 CuSO4 1 mM, 80% loss of activity Trichocladium griseum
3.2.1.28 fructose 10 mM, 45% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
3.2.1.28 lactose 10 mM, 68% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
3.2.1.28 maltose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
3.2.1.28 sucrose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
3.2.1.28 ZnCl2 1 mM, 10% loss of activity Trichocladium griseum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.86
-
 alpha,alpha-trehalose pH 5.5, 60°C Trichocladium griseum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 cytosol
-
 Trichocladium griseum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 BaCl2 1 mM, 1.1fold activation Trichocladium griseum
3.2.1.28 CaCl2 5 mM, 2.9fold activation Trichocladium griseum
3.2.1.28 CoCl2 1 mM, 1.7fold activation Trichocladium griseum
3.2.1.28 MgCl2 10 mM, 1.2fold activation Trichocladium griseum
3.2.1.28 MnCl2 7.5 mM, 4.2fold activation Trichocladium griseum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 12000
-
 3 * 12000, SDS-PAGE Trichocladium griseum
3.2.1.28 360000
-
 gel filtration Trichocladium griseum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Trichocladium griseum
-
 var. therrnoidea
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein contains 12% carbohydrate Trichocladium griseum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Trichocladium griseum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 mycelium
-
 Trichocladium griseum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 106.7
-
 pH 5.5, 60°C, substrate: alpha,alpha-trehalose Trichocladium griseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Trichocladium griseum beta-D-glucose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 trimer 3 * 12000, SDS-PAGE Trichocladium griseum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 60
-
-
 Trichocladium griseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.5
-
-
 Trichocladium griseum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.28 Trichocladium griseum electrofocusing
-
 4