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Literature summary extracted from

  • Qiu, J.; Han, H.; Sun, B.; Chen, L.; Yu, C.; Peng, R.; Yao, Q.
    Residue mutations of xylanase in Aspergillus kawachii alter its optimum pH (2016), Microbiol. Res., 182, 1-7.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 dithiothreitol
-
 Aspergillus luchuensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Pichia pastoris Aspergillus luchuensis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 D48N/T64S shift in pH optimum from 2.0 to 5.0 Aspergillus luchuensis
3.2.1.8 T64S shift in pH optimum from 2.0 to 3.8 Aspergillus luchuensis

General Stability

EC Number General Stability Organism
3.2.1.8 enzyme is strongly resistant to protease digestion. After treatment at 37°C for 30 min with pepsin or trypsin, the enzyme retains more than 90% of the maximum xylanase activity Aspergillus luchuensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ partial inhibition Aspergillus luchuensis
3.2.1.8 Mn2+ more than 50% inhibition Aspergillus luchuensis
3.2.1.8 SDS more than 50% inhibition Aspergillus luchuensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan mutant T64S, Km value 10 mg/ml, pH 3.8, 45°C Aspergillus luchuensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Al3+ slight activation Aspergillus luchuensis
3.2.1.8 Ca2+ slight activation Aspergillus luchuensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 23000
-
-
 Aspergillus luchuensis
3.2.1.8 23300
-
-
 Aspergillus luchuensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus luchuensis P33557
-
-
3.2.1.8 Aspergillus luchuensis NBRC 4308 P33557
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 350
-
 wild-type, pH 3.8, 45°C Aspergillus luchuensis
3.2.1.8 585
-
 mutant T64S, presence of Al3+, pH 3.8, 45°C Aspergillus luchuensis
3.2.1.8 603
-
 mutant T64S, presence of Ca2+, pH 3.8, 45°C Aspergillus luchuensis
3.2.1.8 687
-
 mutant T64S, presence of dithiothreitol, pH 3.8, 45°C Aspergillus luchuensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Aspergillus luchuensis NBRC 4308 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 23000, SDS-PAGE, x * 23300, calculated Aspergillus luchuensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynC
-
 Aspergillus luchuensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 45
-
-
 Aspergillus luchuensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 40
-
 mutant T64S, 30 min, 77% resiudal activity Aspergillus luchuensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 2
-
 wild-type Aspergillus luchuensis
3.2.1.8 3.8
-
 mutant T64S Aspergillus luchuensis
3.2.1.8 5
-
 mutant D48N/T64S Aspergillus luchuensis