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Literature summary extracted from

  • Itagaki, E.
    Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Purification and characterization (1986), J. Biochem., 99, 815-824.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.54 0.0004
-
 progesterone
-
 Ilyonectria destructans
1.14.13.54 0.0043
-
 NADPH with progesterone and O2 as cosubstrates Ilyonectria destructans
1.14.13.54 0.07
-
 O2 with progesterone and NADPH as cosubstrates Ilyonectria destructans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.54 56000
-
 2 * 56000, SDS-PAGE Ilyonectria destructans
1.14.13.54 116000
-
 gel filtration, gel electrophoresis Ilyonectria destructans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.54 Ilyonectria destructans
-
 ATTC 11011
-
1.14.13.54 Ilyonectria destructans ATTC 11011
-
 ATTC 11011
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.54
-
 Ilyonectria destructans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.14.13.54 -80°C, Tris buffer, stable for more than 6 months, enzyme in crude extract Ilyonectria destructans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.54 1,4-Pregnadiene-17alpha,21-diol-3,11,20-trione + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 11beta-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 11beta-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
1.14.13.54 17alpha-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans androstenedione + acetate + NADP+ + H2O
-
 ?
1.14.13.54 17alpha-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 androstenedione + acetate + NADP+ + H2O
-
 ?
1.14.13.54 18-Hydroxydeoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 21-Deoxycortisol + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 Aldosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 androstenedione + NADPH + O2
-
 Ilyonectria destructans 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
 ?
1.14.13.54 androstenedione + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
 ?
1.14.13.54 Corticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 Cortisol + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 Cortisone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 deoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
1.14.13.54 deoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
1.14.13.54 progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
 ?
1.14.13.54 progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans ATTC 11011 Testosterone acetate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.54 dimer 2 * 56000, SDS-PAGE Ilyonectria destructans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.54 7.8
-
 progesterone monooxygenase reaction Ilyonectria destructans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.54 6 9 pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, progesterone monooxygenase activity Ilyonectria destructans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.54 FAD enzyme contains 1.6 mol of FAD per mol of enzyme Ilyonectria destructans