EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.99.2 | synthesis | engineering of a Bacillus subtilis strain that forms significant amounts of pyridoxine via a non-native deoxyxylulose 5'-phosphate-(DXP)-dependent vitamin B6 pathway. The pathway consists of the 4-hydroxy-L-threonine-phosphate dehydrogenase PdxA, the pyridoxine 5'-phosphate synthase PdxJ and the native DXP synthase, Dxs. Bacteria are adapted to tolerate the antimetabolite 4-HO-Thro in order to allow feeding of high amounts of 4-hydroxy-threonine (4-HO-Thr) that can be converted to pyridoxine. The adapted bacteria produce 2834 mg/l pyridoxine from 4-HO-Thr while the wild-type parent produced 12 mg/l pyridoxine. Identification of a better combination of pdxA and pdxJ alleles results in production of 65 mg/l pyridoxine | Sinorhizobium meliloti |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.99.2 | Sinorhizobium meliloti | - |
- |
- |
2.6.99.2 | Sinorhizobium meliloti IFO14782 | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.99.2 | PdxJ | - |
Sinorhizobium meliloti |