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Literature summary extracted from

  • Abdul Wahab, M.; Jonet, M.; Illias, R.
    Thermostability enhancement of xylanase Aspergillus fumigatus RT-1 (2016), J. Mol. Catal. B, 134, 154-163.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 A60D increase in thermostability and in optimum temperature Aspergillus fumigatus
3.2.1.8 Q47P/S159R increase in thermostability and in optimum temperature Aspergillus fumigatus
3.2.1.8 S68R increase in thermostability and in optimum temperature Aspergillus fumigatus
3.2.1.8 T16A/T39I/L176Q increase in thermostabilityand in optimum temperature Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan Km value for wild-type 12.9 mg/ml, mutant T16A/T39I/L176Q 8.9 mg/ml, mutant S68R 12.2 mg/ml, mutant A60D 14.2 mg/ml, mutant Q47P/S159R 11.75 mg/ml, respectively, pH 5.0, 50°C Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 23000
-
 x * 23000, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus fumigatus E0X4B3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 Aspergillus fumigatus xylo-oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 23000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynG1
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
 wild-type and mutants S68R and Q47P/S159R Aspergillus fumigatus
3.2.1.8 60
-
 mutant A60D Aspergillus fumigatus
3.2.1.8 70
-
 mutant T16A/T39I/L176Q Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 1 h, complete loss of activity for wild-type, 46% residual activity for mutant T16A/T39I/L176Q, 33% for mutant A60D, 25.8% for mutant S68R, 23.8% for mutant Q47P/S159R Aspergillus fumigatus