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Literature summary extracted from

  • Cerioli, L.; Planchestainer, M.; Cassidy, J.; Tessaro, D.; Paradisi, F.
    Characterization of a novel amine transaminase from Halomonas elongata (2015), J. Mol. Catal. B, 120, 141-150.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.6.1.B16 in 1 M KCl, 50% residual activity after 24 h Halomonas elongata
2.6.1.B16 in 1 M NaCl, 40% residual activity after 24 h Halomonas elongata
2.6.1.B16 the enzyme at concentrations greater than 5mM of cofactor pyridoxal 5'-phosphate loses the activity significantly faster than 0.1mM, confirming the destabilization effect Halomonas elongata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.B16 0.56
-
 pyruvate pH 8.0, 37°C Halomonas elongata
2.6.1.B16 2.57
-
 (S)-1-phenylethylamine pH 8.0, 37°C Halomonas elongata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.B16 54000
-
 2 * 54000, SDS-PAGE Halomonas elongata
2.6.1.B16 109000
-
 gel filtration Halomonas elongata

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.6.1.B16 DMSO 20%, 27% residual activity after 22 h Halomonas elongata
2.6.1.B16 Ethanol 20%, 25% residual activity after 22 h Halomonas elongata
2.6.1.B16 isopropanol 20%, 16% residual activity after 22 h Halomonas elongata
2.6.1.B16 Methanol 20%, 47% residual activity after 22 h Halomonas elongata
2.6.1.B16 tert-Butanol 20%, 20% residual activity after 22 h Halomonas elongata

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B16 Halomonas elongata E1V913
-
-
2.6.1.B16 Halomonas elongata DSM 2581 E1V913
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.B16 0.58
-
 substrate benzaldehyde, cosubstrate isopropylamine, pH 8.0, 37°C Halomonas elongata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B16 2-phenylpropionaldehyde + (S)-1-phenylethylamine 67% conversion of 2-phenylpropionaldehyde Halomonas elongata 2-phenylpropylamine + acetophenone
-
 ?
2.6.1.B16 2-phenylpropionaldehyde + (S)-1-phenylethylamine 67% conversion of 2-phenylpropionaldehyde Halomonas elongata DSM 2581 2-phenylpropylamine + acetophenone
-
 ?
2.6.1.B16 benzaldehyde + ortho-xylylenediamine 95% conversion of benzaldehyde Halomonas elongata benzylamine + ? conversion rate 90-95% ?
2.6.1.B16 benzaldehyde + ortho-xylylenediamine 95% conversion of benzaldehyde Halomonas elongata DSM 2581 benzylamine + ? conversion rate 90-95% ?
2.6.1.B16 cinnamaldehyde + (S)-1-phenylethylamine 56% conversion of cinnamaldehyde Halomonas elongata (2E)-3-phenylprop-2-en-1-amine + acetophenone
-
 ?
2.6.1.B16 cinnamaldehyde + (S)-1-phenylethylamine 56% conversion of cinnamaldehyde Halomonas elongata DSM 2581 (2E)-3-phenylprop-2-en-1-amine + acetophenone
-
 ?
2.6.1.B16 L-alanine + glyoxylate 41% conversion of alanine, 97% conversion of glyoxylate Halomonas elongata pyruvate + glycine
-
 ?
2.6.1.B16 L-alanine + glyoxylate 41% conversion of alanine, 97% conversion of glyoxylate Halomonas elongata DSM 2581 pyruvate + glycine
-
 ?
2.6.1.B16 propanal + (S)-1-phenylethylamine 55% conversion of propanal Halomonas elongata propylamine + acetophenone
-
 ?
2.6.1.B16 pyruvate + (S)-1-phenylethylamine 97% conversion of (S)-1-phenylethylamine, less than 5% conversion of (R)-1-phenylethylamine Halomonas elongata L-alanine + acetophenone
-
 ?
2.6.1.B16 pyruvate + benzylamine 64% conversion of isopropylamine Halomonas elongata L-alanine + benzaldehyde
-
 ?
2.6.1.B16 pyruvate + glycine 97% conversion of pyruvate Halomonas elongata L-alanine + glyoxylate
-
 ?
2.6.1.B16 pyruvate + glycine 97% conversion of pyruvate Halomonas elongata DSM 2581 L-alanine + glyoxylate
-
 ?
2.6.1.B16 pyruvate + isopropylamine 41% conversion of isopropylamine Halomonas elongata L-alanine + acetone
-
 ?
2.6.1.B16 pyruvate + L-serine 19% conversion of serine Halomonas elongata L-alanine + 3-hydroxy-2-oxopropanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.B16 dimer 2 * 54000, SDS-PAGE Halomonas elongata

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B16 HELO 1904
-
 Halomonas elongata
2.6.1.B16 HEWT
-
 Halomonas elongata
2.6.1.B16 SpuC
-
 Halomonas elongata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.B16 50
-
-
 Halomonas elongata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.B16 35
-
 24 h, stable up to 35°C Halomonas elongata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.B16 0.094
-
 (S)-1-phenylethylamine pH 8.0, 37°C Halomonas elongata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B16 10
-
-
 Halomonas elongata

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B16 pyridoxal 5'-phosphate
-
 Halomonas elongata