EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.142 | structures of barley limit dextrinase and active-site mutants in complex with natural substrates, products and substrate analogues. Avoidance of alpha-1,4-hydrolytic activity and strong preference for alpha-1,6-hydrolytic activity seems to be based on the strong interaction to Trp512 and Phe553 on each flank of the catalytic cleft, which will keep non-branched polysaccharides out of the reach of the catalytic nucleophile and acid-base catalyst | Hordeum vulgare |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.142 | E510A | 0.0004% residual activity with pullulan | Hordeum vulgare |
3.2.1.142 | M440G | 2.6fold decrease in catalytic efficiency | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.142 | additional information | - |
pullulan | Km value of wild-type 0.16 mg/ml, mutant M440G 0.15 mg/ml, respectively, 37°C, pH not specified in the publication | Hordeum vulgare | |
3.2.1.142 | additional information | - |
amylopectin | Km value of wild-type 6.9 mg/ml, mutant M440G 4.4 mg/ml, respectively, 37°C, pH not specified in the publication | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.142 | Hordeum vulgare | O48541 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.142 | amylopectin + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.2.1.142 | pullulan + H2O | - |
Hordeum vulgare | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.142 | HvLD99 | - |
Hordeum vulgare |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.142 | 3.9 | - |
amylopectin | mutant M440G, 37°C, pH not specified in the publication | Hordeum vulgare | |
3.2.1.142 | 15.6 | - |
amylopectin | wild-type, 37°C, pH not specified in the publication | Hordeum vulgare | |
3.2.1.142 | 72 | - |
pullulan | mutant M440G, 37°C, pH not specified in the publication | Hordeum vulgare | |
3.2.1.142 | 78 | - |
pullulan | wild-type, 37°C, pH not specified in the publication | Hordeum vulgare |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.142 | additional information | - |
amylopectin | kcat/Km value of wild-type 2.3 ml/mg/s, mutant M440G 0.9 ml/mg/s, respectively, 37°C, pH not specified in the publication | Hordeum vulgare | |
3.2.1.142 | additional information | - |
pullulan | kcat/Km value of wild-type 488 ml/mg/s, mutant M440G 480 ml/mg/s, respectively, 37°C, pH not specified in the publication | Hordeum vulgare |