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Literature summary extracted from

  • Witschel, M.; Nagel, S.; Egli, T.
    Identification and characterization of the two-enzyme system catalyzing oxidation of EDTA in the EDTA-degrading bacterial strain DSM 9103 (1997), J. Bacteriol., 179, 6937-6943.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.33 Chelativorans multitrophicus A7LKG9
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-
1.14.14.33 Chelativorans multitrophicus DSM 9103 A7LKG9
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-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.33 1.6
-
pH 8.0, 30°C Chelativorans multitrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 100% activity in presence of Mg2+, 53% in presence of Zn2+, 35% in presence of Mn2+, 19% in presence of Co2+, 11% in presence of Cu2+ Chelativorans multitrophicus ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O
-
?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 100% activity in presence of Mg2+, 53% in presence of Zn2+, 35% in presence of Mn2+, 19% in presence of Co2+, 11% in presence of Cu2+ Chelativorans multitrophicus DSM 9103 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O
-
?
1.14.14.33 additional information the EDTA-oxidizing enzyme complex accepts EDTA as a substrate only when it is complexed with Mg2+, Zn2+, Mn2+, Co2+, or Cu2+. It catalyzes the removal of acetyl groups from several other aminopolycarboxylic acids that possess three or more acetyl groups Chelativorans multitrophicus ?
-
?
1.14.14.33 additional information the EDTA-oxidizing enzyme complex accepts EDTA as a substrate only when it is complexed with Mg2+, Zn2+, Mn2+, Co2+, or Cu2+. It catalyzes the removal of acetyl groups from several other aminopolycarboxylic acids that possess three or more acetyl groups Chelativorans multitrophicus DSM 9103 ?
-
?

General Information

EC Number General Information Comment Organism
1.14.14.33 physiological function the first two steps of the catabolic pathway for EDTA consist of the sequential oxidative removal of two acetyl groups, resulting in the formation of glyoxylate. Ethylenediaminetriacetate (ED3A) is formed as an intermediate and N,N'-ethylenediaminediacetate is the end product. The enzyme complex consists of a monooxygenase, which catalyzes the cleavage of EDTA and ED3A while consuming oxygen and reduced flavin mononucleotide FMNH2, and an NADH2:FMN oxidoreductase that provides FMNH2 for the monooxygenase. The oxidoreductase can be replaced by other NADH2:FMN oxidoreductases Chelativorans multitrophicus