EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.33 | Chelativorans multitrophicus | A7LKG9 | - |
- |
1.14.14.33 | Chelativorans multitrophicus DSM 9103 | A7LKG9 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.33 | 1.6 | - |
pH 8.0, 30°C | Chelativorans multitrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.33 | ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | 100% activity in presence of Mg2+, 53% in presence of Zn2+, 35% in presence of Mn2+, 19% in presence of Co2+, 11% in presence of Cu2+ | Chelativorans multitrophicus | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | - |
? | |
1.14.14.33 | ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | 100% activity in presence of Mg2+, 53% in presence of Zn2+, 35% in presence of Mn2+, 19% in presence of Co2+, 11% in presence of Cu2+ | Chelativorans multitrophicus DSM 9103 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | - |
? | |
1.14.14.33 | additional information | the EDTA-oxidizing enzyme complex accepts EDTA as a substrate only when it is complexed with Mg2+, Zn2+, Mn2+, Co2+, or Cu2+. It catalyzes the removal of acetyl groups from several other aminopolycarboxylic acids that possess three or more acetyl groups | Chelativorans multitrophicus | ? | - |
? | |
1.14.14.33 | additional information | the EDTA-oxidizing enzyme complex accepts EDTA as a substrate only when it is complexed with Mg2+, Zn2+, Mn2+, Co2+, or Cu2+. It catalyzes the removal of acetyl groups from several other aminopolycarboxylic acids that possess three or more acetyl groups | Chelativorans multitrophicus DSM 9103 | ? | - |
? |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.33 | physiological function | the first two steps of the catabolic pathway for EDTA consist of the sequential oxidative removal of two acetyl groups, resulting in the formation of glyoxylate. Ethylenediaminetriacetate (ED3A) is formed as an intermediate and N,N'-ethylenediaminediacetate is the end product. The enzyme complex consists of a monooxygenase, which catalyzes the cleavage of EDTA and ED3A while consuming oxygen and reduced flavin mononucleotide FMNH2, and an NADH2:FMN oxidoreductase that provides FMNH2 for the monooxygenase. The oxidoreductase can be replaced by other NADH2:FMN oxidoreductases | Chelativorans multitrophicus |