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Literature summary extracted from

  • Niwa, R.; Niimi, T.; Honda, N.; Yoshiyama, M.; Itoyama, K.; Kataoka, H.; Shinoda, T.
    Juvenile hormone acid O-methyltransferase in Drosophila melanogaster (2008), Insect Biochem. Mol. Biol., 38, 714-720.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.325 expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.325 32000
-
 x * 32000, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.325 Drosophila melanogaster Q9VJK8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.325 HiTrap Ni-chelating column chromatography, and gel filtration Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.325 corpora allata
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.325 S-adenosyl-L-methionine + (2E,6E)-farnesoate
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
2.1.1.325 S-adenosyl-L-methionine + juvenile hormone III acid
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?
2.1.1.325 S-adenosyl-L-methionine + laurate
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.325 S-adenosyl-L-methionine + palmitate
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.325 ? x * 32000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.1.1.325 JHAMT
-
 Drosophila melanogaster
2.1.1.325 juvenile hormone acid O-methyltransferase
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.325 0.000683
-
 palmitate at pH 7.5 and 25°C Drosophila melanogaster
2.1.1.325 0.0017
-
 laurate at pH 7.5 and 25°C Drosophila melanogaster
2.1.1.325 0.128
-
 juvenile hormone III acid at pH 7.5 and 25°C Drosophila melanogaster
2.1.1.325 0.168
-
 (2E,6E)-farnesoate at pH 7.5 and 25°C Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.1.1.325 physiological function enzyme overexpression results in a pharate adult lethal phenotype suggesting that the temporal regulation of the enzyme is critical for Drosophila development Drosophila melanogaster