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Literature summary extracted from

  • Guo, Y.; Yan, Q.; Yang, Y.; Yang, S.; Liu, Y.; Jiang, Z.
    Expression and characterization of a novel beta-glucosidase, with transglycosylation and exo-beta-1,3-glucanase activities, from Rhizomucor miehei (2015), Food Chem., 175, 431-438.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Rhizomucor miehei
3.2.1.58 expression in Escherichia coli Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.17
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.92
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 1.26
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 1.5 2 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 2.29
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 2.32
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 3.7
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 4.15
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.17
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.92
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 1.26
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 1.5 2 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 2.29
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 2.32
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 3.7
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 4.15
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 66400
-
 gel filtration Rhizomucor miehei
3.2.1.21 76500
-
 SDS-PAGE Rhizomucor miehei
3.2.1.58 66400
-
 gel filtration Rhizomucor miehei
3.2.1.58 76500
-
 SDS-PAGE Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rhizomucor miehei A0A0B4RUW2
-
-
3.2.1.58 Rhizomucor miehei A0A0B4RUW2 cf. EC 3.2.1.21
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 19.1
-
 substrate laminaran, pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 19.1
-
 substrate laminaran, pH 5.0, 50°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-glucose 2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside ?
3.2.1.21 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-xylose 3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Rhizomucor miehei 2 D-glucose 62% of the activity with laminaribiose ?
3.2.1.21 cellotetraose + H2O
-
 Rhizomucor miehei D-glucose + ? 64% of the activity with laminaribiose ?
3.2.1.21 cellotriose + H2O
-
 Rhizomucor miehei D-glucose + ? 67% of the activity with laminaribiose ?
3.2.1.21 gentobiose + H2O
-
 Rhizomucor miehei 2 D-glucose 52% of the activity with laminaribiose ?
3.2.1.21 laminaran + H2O 67% of the activity with laminaribiose Rhizomucor miehei D-glucose + gentiobiose + gentiotriose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Rhizomucor miehei 2 D-glucose
-
 ?
3.2.1.21 laminaritriose + H2O
-
 Rhizomucor miehei D-glucose + ? 97% of the activity with laminaribiose ?
3.2.1.21 additional information isoform Bglu3B exhibits a broad range of substrate specificity, catalyzing the cleavage of beta-1,2, beta-1,3, beta-1,4 and beta-1,6 linkages, in various oligosaccharides, to liberate glucose, e.g. reaction of EC 3.2.1.58. Bglu3B shows relatively high activity toward laminaran and transglycosylation activity, enabling gentiobiose production. No substrates: 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, lactose, barley beta-glucan, Avicel, carboxymethyl cellulose, lichenan, birchwood xylan and chitin Rhizomucor miehei ?
-
 ?
3.2.1.58 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-glucose 2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside ?
3.2.1.58 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-xylose 3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside ?
3.2.1.58 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + D-glucose
-
 ?
3.2.1.58 cellobiose + H2O
-
 Rhizomucor miehei 2 D-glucose 62% of the activity with laminaribiose ?
3.2.1.58 cellotetraose + H2O
-
 Rhizomucor miehei D-glucose + ? 64% of the activity with laminaribiose ?
3.2.1.58 cellotriose + H2O
-
 Rhizomucor miehei D-glucose + ? 67% of the activity with laminaribiose ?
3.2.1.58 gentobiose + H2O
-
 Rhizomucor miehei 2 D-glucose 52% of the activity with laminaribiose ?
3.2.1.58 laminaran + H2O 67% of the activity with laminaribiose Rhizomucor miehei D-glucose + gentiobiose + gentiotriose
-
 ?
3.2.1.58 laminaribiose + H2O
-
 Rhizomucor miehei 2 D-glucose
-
 ?
3.2.1.58 laminaritriose + H2O
-
 Rhizomucor miehei D-glucose + ? 97% of the activity with laminaribiose ?
3.2.1.58 additional information isoform Bglu3B exhibits a broad range of substrate specificity, catalyzing the cleavage of beta-1,2, beta-1,3, beta-1,4 and beta-1,6 linkages, in various oligosaccharides, to liberate glucose. Bglu3B shows relatively high activity toward laminaran and transglycosylation activity, enabling gentiobiose production. No substrates: 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, lactose, barley beta-glucan, Avicel, carboxymethyl cellulose, lichenan, birchwood xylan and chitin Rhizomucor miehei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bglu3B
-
 Rhizomucor miehei
3.2.1.58 Bglu3B
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Rhizomucor miehei
3.2.1.58 50
-
-
 Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.033
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.043
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.056
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.059
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.067
-
 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.071
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.077
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.078
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.033
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.043
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.056
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.059
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.067
-
 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.071
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.077
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.078
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Rhizomucor miehei
3.2.1.58 5
-
-
 Rhizomucor miehei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 0.012
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.013
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.014
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.033
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.044
-
 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.062
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.063
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.21 0.42
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.012
-
 cellobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.013
-
 gentobiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.014
-
 Laminaran pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.033
-
 laminaritriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.044
-
 cellotriose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.062
-
 laminaribiose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.063
-
 cellotetraose pH 5.0, 50°C Rhizomucor miehei
3.2.1.58 0.42
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Rhizomucor miehei