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Literature summary extracted from

  • Graczer, E.; Pallo, A.; Olah, J.; Szimler, T.; Konarev, P.V.; Svergun, D.I.; Merli, A.; Zavodszky, P.; Weiss, M.S.; Vas, M.
    Glutamate 270 plays an essential role in K+-activation and domain closure of Thermus thermophilus isopropylmalate dehydrogenase (2015), FEBS Lett., 589, 240-245.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.85 mutant enzyme E270A, hanging drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.1 M MOPS/KOH pH 7.6, and 10% (v/v) ethanol Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 E270A the mutant exhibits largely reduced (about 1%) catalytic activity and negligible activation by K+ compared to the wild type enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.016
-
 (2R,3S)-3-isopropylmalate wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.032
-
 (2R,3S)-3-isopropylmalate mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.29
-
 NAD+ wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.66
-
 NAD+ mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 K+ activates Thermus thermophilus
1.1.1.85 Mn2+ activates Thermus thermophilus
1.1.1.85 NH4+ activates Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Thermus thermophilus
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus Q5SIY4
-
-
1.1.1.85 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SIY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Thermus thermophilus
1.1.1.85 isopropylmalate dehydrogenase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.033
-
 (2R,3S)-3-isopropylmalate mutant enzyme E270A, in the presence of K+, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 3.97
-
 (2R,3S)-3-isopropylmalate wild type enzyme, in the presence of K+, at pH 7.6 and 20°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Thermus thermophilus