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Literature summary extracted from

  • Kimura, T.; Shirakawa, R.; Yaoita, N.; Hayashi, T.; Nagano, K.; Horiuchi, H.
    The antimalarial drugs chloroquine and primaquine inhibit pyridoxal kinase, an essential enzyme for vitamin B6 production (2014), FEBS Lett., 588, 3673-3676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.35 Chloroquine about 40% inhibition at 1 mM Plasmodium vivax
2.7.1.35 additional information not inhibited by chloroquine Homo sapiens
2.7.1.35 additional information not inhibited by chloroquine Trypanosoma cruzi
2.7.1.35 primaquine competitive inhibition, complete inhibition at 0.75 mM Homo sapiens
2.7.1.35 primaquine competitive inhibition, complete inhibition at 1 mM Plasmodium vivax
2.7.1.35 primaquine competitive inhibition, about 70% inhibition at 1 mM Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.35 0.00122
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Homo sapiens
2.7.1.35 0.0153
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Plasmodium vivax
2.7.1.35 0.0216
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Trypanosoma cruzi
2.7.1.35 0.0739
-
 pyridoxal in the presence of Na+, at pH 7.0 and 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 K+ activates Homo sapiens
2.7.1.35 K+ activates Trypanosoma cruzi
2.7.1.35 K+ activates Plasmodium vivax
2.7.1.35 Na+ activates Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.35 35000
-
 x * 35000, SDS-PAGE Homo sapiens
2.7.1.35 35000
-
 x * 35000, SDS-PAGE Trypanosoma cruzi
2.7.1.35 35000
-
 x * 35000, SDS-PAGE Plasmodium vivax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.35 ATP + pyridoxal Homo sapiens
-
 ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal Trypanosoma cruzi
-
 ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal Plasmodium vivax
-
 ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal Plasmodium vivax Salvador I
-
 ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal Trypanosoma cruzi CL Brener
-
 ADP + pyridoxal 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Homo sapiens
-
-
-
2.7.1.35 Plasmodium vivax A5K1Z8
-
-
2.7.1.35 Plasmodium vivax Salvador I A5K1Z8
-
-
2.7.1.35 Trypanosoma cruzi Q4D946
-
-
2.7.1.35 Trypanosoma cruzi CL Brener Q4D946
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 Ni-NTA agarose column chromatography Homo sapiens
2.7.1.35 Ni-NTA agarose column chromatography Trypanosoma cruzi
2.7.1.35 Ni-NTA agarose column chromatography Plasmodium vivax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.35 lung
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Trypanosoma cruzi ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Plasmodium vivax ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Plasmodium vivax Salvador I ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Trypanosoma cruzi CL Brener ADP + pyridoxal 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 ? x * 35000, SDS-PAGE Homo sapiens
2.7.1.35 ? x * 35000, SDS-PAGE Trypanosoma cruzi
2.7.1.35 ? x * 35000, SDS-PAGE Plasmodium vivax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.35 0.015
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Trypanosoma cruzi
2.7.1.35 0.073
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Plasmodium vivax
2.7.1.35 0.12
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Homo sapiens
2.7.1.35 0.23
-
 pyridoxal in the presence of Na+, at pH 7.0 and 30°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.35 0.0572
-
 primaquine at pH 7.0 and 30°C Homo sapiens