Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cross, P.J.; Parker, E.J.
    Allosteric inhibitor specificity of Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (2013), FEBS Lett., 587, 3063-3068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 gene aroG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain (DE3) Star Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 H29A site-directed mutagenesis, the mutant is inhibited by both L-Tyr and L-Phe Thermotoga maritima
2.5.1.54 H29S/S31H site-directed mutagenesis, the mutant is inhibited to a greater extent by L-Phe than L-Tyr Thermotoga maritima
2.5.1.54 S31G site-directed mutagenesis, the mutation severely reduces inhibition by L-Tyr Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 L-phenylalanine involved in allosteric regulation of the enzyme, inhibits the wild-type enzyme, and enzyme mutants H29A and H29S/S31H Thermotoga maritima
2.5.1.54 L-tyrosine allosteric regulation of the enzyme is mediated by L-Tyr binding to a discrete ACT regulatory domain appended to a core catalytic (beta/alpha)8 barrel, highly reduced inhibition of enzyme mutant S31G. Comparison of the position of the regulatory ACT domain of TmaDAH7PS in the unbound and L-Tyr-bound conformations, overview Thermotoga maritima
2.5.1.54 additional information small angle X-ray (SAXS) scattering analysis Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information Michaelis-Menten kinetics Thermotoga maritima
2.5.1.54 0.00485
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima
2.5.1.54 0.0128
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima
2.5.1.54 0.013
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima
2.5.1.54 0.021
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 0.028
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima
2.5.1.54 0.03
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 0.033
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima
2.5.1.54 0.039
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Thermotoga maritima
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Thermotoga maritima Q9WYH8 gene aroF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Thermotoga maritima 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
-
 Thermotoga maritima
2.5.1.54 DAH7PS
-
 Thermotoga maritima
2.5.1.54 phospho-2-dehydro-3-deoxyheptonate aldolase UniProt Thermotoga maritima
2.5.1.54 TmaDAH7PS
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 11.7
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima
2.5.1.54 11.7
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima
2.5.1.54 14.1
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima
2.5.1.54 14.1
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima
2.5.1.54 14.3
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 14.3
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima
2.5.1.54 17.5
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 17.5
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.3
-
 assay at Thermotoga maritima

General Information

EC Number General Information Comment Organism
2.5.1.54 metabolism the enzyme catalyses the first step of the shikimate pathway for the biosynthesis of aromatic amino acids Thermotoga maritima
2.5.1.54 physiological function allosteric regulation of the enzyme is mediated by L-Tyr binding to a discrete ACT regulatory domain appended to a core catalytic (beta/alpha)8 barrel Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.54 360
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima
2.5.1.54 430
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima
2.5.1.54 500
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29S/S31H Thermotoga maritima
2.5.1.54 580
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 700
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant H29A Thermotoga maritima
2.5.1.54 900
-
 D-erythrose 4-phosphate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima
2.5.1.54 1400
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, mutant S31G Thermotoga maritima
2.5.1.54 2400
-
 phosphoenolpyruvate pH 7.3, temperature not specified in the publication, wild-type enzyme Thermotoga maritima