Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zamporlini, F.; Ruggieri, S.; Mazzola, F.; Amici, A.; Orsomando, G.; Raffaelli, N.
    Novel assay for simultaneous measurement of pyridine mononucleotides synthesizing activities allows dissection of the NAD+ biosynthetic machinery in mammalian cells (2014), FEBS J., 281, 5104-5119.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.22 analysis simple, fast and sensitive coupled fluorometric assay that enables simultaneous determination of nicotinamide phosphoribosyltransferase EC 2.4.2.12, quinolinate phosphoribosyltransferase EC 2.4.2.19, nicotinate phosphoribosyltransferase EC 6.3.4.21 and nicotinamide riboside kinase in whole-cell extracts and biological fluids yielding an overall picture of the tissue/cell-specific distribution of the activities of the various enzymes Homo sapiens
2.7.1.173 diagnostics screening of tumors for NRK activity is necessary for the design of efficient NamPRT-targeted antitumor therapy Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.22 Homo sapiens
-
-
-
2.7.1.173 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.22 blood plasma
-
 Homo sapiens
-
2.7.1.22 kidney and liver, highest eypression Homo sapiens
-
2.7.1.22 liver and kidney, highest eypression Homo sapiens
-
2.7.1.22 additional information presence of quinolinate phosphoribosyltransferase and nicotinamide riboside kinase in all examined tissues/cell lines Homo sapiens
-
2.7.1.173 brain
-
 Homo sapiens
-
2.7.1.173 heart
-
 Homo sapiens
-
2.7.1.173 kidney
-
 Homo sapiens
-
2.7.1.173 liver
-
 Homo sapiens
-
2.7.1.173 lung
-
 Homo sapiens
-
2.7.1.173 additional information ubiquitous expression of nicotinamide riboside kinase in tissues and cell lines Homo sapiens
-
2.7.1.173 skeletal muscle
-
 Homo sapiens
-
2.7.1.173 spleen
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.173 ATP + beta-D-ribosylnicotinate
-
 Homo sapiens ADP + nicotinate beta-D-ribonucleotide
-
 ?
2.7.1.173 additional information development of a highly sensitive, rapid and specific coupled enzymatic assay that allows simultaneous determination of NRK activitiy, as well as of nicotinic acid phosphoribosyltransferase, quinolinic acid phosphoribosyltransferase, and nicotinamide phosphoribosyltransferase activities, in various biological samples of various origins, evaluation and optimization using liver extracts, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.173 NR kinase
-
 Homo sapiens
2.7.1.173 Nrk
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.173 metabolism distinct metabolic routes, starting from various precursors, are known to support NAD+ biosynthesis with tissue/cell-specific efficiencies, probably reflecting differential expression of the corresponding rate-limiting enzymes, i.e. nicotinamide phosphoribosyltransferase, quinolinate phosphoribosyltransferase, nicotinate phosphoribosyltransferase and nicotinamide riboside kinase Homo sapiens
2.7.1.173 physiological function nicotinamide riboside is a relevant NAD+ precursor Homo sapiens