Literature summary extracted from

Pallo, A.; Olah, J.; Graczer, E.; Merli, A.; Zavodszky, P.; Weiss, M.S.; Vas, M.
Structural and energetic basis of isopropylmalate dehydrogenase enzyme catalysis (2014), FEBS J., 281, 5063-5076.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.85	in complex with Mn2+, (2R,3S)-3-isopropylmalate, and NADH, hanging drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.1 M MOPS/KOH, pH 7.6, and 10% (v/v) ethanol	Thermus thermophilus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.85	Mn2+	required	Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.85	(2R,3S)-3-isopropylmalate + NAD+	Thermus thermophilus	-	(2S)-2-isopropyl-3-oxosuccinate + NADH + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.85	Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.85	(2R,3S)-3-isopropylmalate + NAD+	-	Thermus thermophilus	(2S)-2-isopropyl-3-oxosuccinate + NADH + H+	-	r

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.85	IPMDH	-	Thermus thermophilus
1.1.1.85	isopropylmalate dehydrogenase	-	Thermus thermophilus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.85	NAD+	-	Thermus thermophilus

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Release 2023.1 (January 2023)