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Literature summary extracted from

  • Preuss, J.; Hort, W.; Lang, S.; Netsch, A.; Rahlfs, S.; Lochnit, G.; Jortzik, E.; Becker, K.; Mayser, P.A.
    Characterization of tryptophan aminotransferase 1 of Malassezia furfur, the key enzyme in the production of indolic compounds by M.furfur (2013), Exp. Dermatol., 22, 736-741.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.27 expression in Escherichia coli Malassezia furfur

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.27 Aminooxyacetate
-
 Malassezia furfur
2.6.1.27 cycloserine
-
 Malassezia furfur

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.27 2.9
-
 2-oxoglutarate pH 8.0, 42°C Malassezia furfur
2.6.1.27 7
-
 L-tryptophan pH 8.0, 42°C Malassezia furfur

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.27 50000
-
-
 Malassezia furfur
2.6.1.27 52500
-
-
 Malassezia furfur

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.27 Malassezia furfur S4UF58
-
-
2.6.1.27 Malassezia furfur CBS 7019 S4UF58
-
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.27 4°C, H2O, stable for around 1 week Malassezia furfur

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.27 L-tryptophan + 2-oxoglutarate
-
 Malassezia furfur (indol-3-yl)pyruvate + L-glutamate
-
 ?
2.6.1.27 L-tryptophan + 2-oxoglutarate
-
 Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + L-glutamate
-
 ?
2.6.1.27 L-tryptophan + 2-oxomethylthiobutanoate 113% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur (indol-3-yl)pyruvate + 2-aminomethylthiobutanoate
-
 ?
2.6.1.27 L-tryptophan + 2-oxomethylthiobutanoate 113% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + 2-aminomethylthiobutanoate
-
 ?
2.6.1.27 L-tryptophan + phenylpyruvate 93% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur (indol-3-yl)pyruvate + L-phenylalanine
-
 ?
2.6.1.27 L-tryptophan + phenylpyruvate 93% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + L-phenylalanine
-
 ?
2.6.1.27 additional information no substrates: amine acceptors glyoxylic acid and hydroxypyruvate, amine donors L-phenylalanine, L-alanine, L-glycine, L-serine, L-histidine Malassezia furfur ?
-
 ?
2.6.1.27 additional information no substrates: amine acceptors glyoxylic acid and hydroxypyruvate, amine donors L-phenylalanine, L-alanine, L-glycine, L-serine, L-histidine Malassezia furfur CBS 7019 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.27 ? x * 50000, SDS-PAGE, x * 52500, calculated Malassezia furfur

Synonyms

EC Number Synonyms Comment Organism
2.6.1.27 Tam1
-
 Malassezia furfur

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.27 40
-
-
 Malassezia furfur

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.27 8
-
-
 Malassezia furfur

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.27 pyridoxal 5'-phosphate activity is highly dependent on the cofactor and increases in presence of additional cofactor Malassezia furfur

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.6.1.27 0.8
-
 pH 8.0, 42°C Malassezia furfur Aminooxyacetate
2.6.1.27 86
-
 pH 8.0, 42°C Malassezia furfur cycloserine