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Literature summary extracted from

  • Terada, T.; Mukae, H.; Ohashi, K.; Hosomi, S.; Mizoguchi, T.; Uehara, K.
    Characterization of an enzyme which catalyzes isomerization and epimerization of D-erythrose 4-phosphate (1985), Eur. J. Biochem., 148, 345-351.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.1 HgCl2 0.1 mM, 100% inhibition of D-ribulose-5-phosphate 3-epimerase activity Bos taurus
5.1.3.1 potassium phosphate 10 mM, 49% inhibition of D-ribulose-5-phosphate 3-epimerase activity Bos taurus
5.1.3.1 Sodium sulfate 20 mM, 65% inhibition of D-ribulose-5-phosphate 3-epimerase activity Bos taurus
5.3.1.34 HgCl2 0.1 mM, 100% inhibition of D-erythrose 4-phosphate isomerase activity Bos taurus
5.3.1.34 pentulose 5-phosphate i.e. enzymatic equilibrium mixture of D-ribulose 5-phosphate and D-xylulose 5-phosphate, 1 mM, 63% inhibition Bos taurus
5.3.1.34 potassium phosphate 10 mM, 45% inhibition of D-erythrose 4-phosphate isomerase activity Bos taurus
5.3.1.34 Sodium sulfate 20 mM, 66% inhibition of D-erythrose 4-phosphate isomerase activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.1 22500
-
 2 * 22500, SDS-PAGE Bos taurus
5.1.3.1 43000
-
 gel filtration Bos taurus
5.3.1.34 22500
-
 2 * 22500, SDS-PAGE Bos taurus
5.3.1.34 43000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.1 Bos taurus
-
-
-
5.3.1.34 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.1
-
 Bos taurus
5.3.1.34
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.1 liver
-
 Bos taurus
-
5.3.1.34 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.1 9.05
-
 substrate D-erythrose 4-phosphate, pH 7.0, 30°C Bos taurus
5.1.3.1 6570
-
 substrate D-ribulose-5-phosphate, pH 7.0, 30°C Bos taurus
5.3.1.34 9.05
-
 substrate D-erythrose 4-phosphate, pH 7.0, 30°C Bos taurus
5.3.1.34 6570
-
 substrate D-ribulose-5-phosphate, pH 7.0, 30°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
5.1.3.1 4°C, 10 mM Bistris/HCl buffer (pH 7.0), stable for about six months without any loss of enzymatic activity Bos taurus
5.3.1.34 4°C, 10 mM Bistris/HCl buffer (pH 7.0), stable for about six months without any loss of enzymatic activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.1 D-erythrose 4-phosphate reaction of EC 5.1.3.1 Bos taurus D-erythrulose 4-phosphate
-
 ?
5.1.3.1 D-ribulose-5-phosphate
-
 Bos taurus D-xylulose 5-phosphate
-
 ?
5.3.1.34 D-erythrose 4-phosphate
-
 Bos taurus D-erythrulose 4-phosphate initially D-threose 4-phosphate is also formed. After 240 min, about 90% of tetrose 4-phosphate is D-erythrulose 4-phosphate, 5% is D-threose 4-phosphate ?
5.3.1.34 D-ribulose-5-phosphate
-
 Bos taurus D-xylulose 5-phosphate strong D-ribulose-5-phosphate 3-epimerase activity, reaction of EC 5.1.3.1 ?
5.3.1.34 D-threose 4-phosphate 130% of the activity with D-erythrose 4-phosphate Bos taurus D-erythrulose 4-phosphate D-threose 4-phosphate isomerizes non-enzymatically more easily than D-erythrose 4-phosphate to D-erythrulose 4-phosphate ?
5.3.1.34 additional information enzyme can catalyze simultaneously both the isomerization and epimerization of D-erythrose 4-phosphate to D-erythrulose 4-phosphate and D-threose 4-phosphate. No isomerase activity with D-glyceraldehyde 3-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate and D-fructose 6-phosphate Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.1 dimer 2 * 22500, SDS-PAGE Bos taurus
5.3.1.34 dimer 2 * 22500, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.1 65
-
 10 min, stable Bos taurus
5.3.1.34 65
-
 10 min, stable Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.1 8.5
-
-
 Bos taurus
5.3.1.34 8.5
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.1 6.5 9.5 22 h, 0°C, stable Bos taurus
5.3.1.34 6.5 9.5 22 h, 0°C, stable Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.1 Bos taurus isoelectric focusing
-
 4.8
5.3.1.34 Bos taurus isoelectric focusing
-
 4.8