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Literature summary extracted from

  • Linke, D.; Lehnert, N.; Nimtz, M.; Berger, R.G.
    An alcohol oxidase of Phanerochaete chrysosporium with a distinct glycerol oxidase activity (2014), Enzyme Microb. Technol., 61-62, 7-12.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.B4 1.9
-
 glycerol at pH 9.0 and 50°C Phanerodontia chrysosporium
1.1.3.13 1.9
-
 glycerol at pH 9.0 and 50°C Phanerodontia chrysosporium
1.1.3.13 10.9
-
 n-Pentanol at pH 9.0 and 50°C Phanerodontia chrysosporium
1.1.3.13 22.5
-
 ethanol at pH 9.0 and 50°C Phanerodontia chrysosporium
1.1.3.13 36.6
-
 methanol at pH 9.0 and 50°C Phanerodontia chrysosporium
1.1.3.13 42.7
-
 1-propanol at pH 9.0 and 50°C Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.B4 75000
-
 2 * 75000, SDS-PAGE Phanerodontia chrysosporium
1.1.3.13 75000
-
 2 * 75000, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.B4 glycerol + O2 Phanerodontia chrysosporium 17% activity compared to methanol glyceraldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.B4 Phanerodontia chrysosporium T2M2J4
-
-
1.1.3.13 Phanerodontia chrysosporium
-
-
-
1.1.3.13 Phanerodontia chrysosporium T2M2J4
-
-
1.1.3.13 Phanerodontia chrysosporium DSMZ 1547
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.B4 Superdex 75 gel filtration Phanerodontia chrysosporium
1.1.3.13 Superdex 75 gel filtration Phanerodontia chrysosporium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.B4 culture condition:lactate-grown cell
-
 Phanerodontia chrysosporium
-
1.1.3.13 culture condition:lactate-grown cell
-
 Phanerodontia chrysosporium
-
1.1.3.13 mycelium
-
 Phanerodontia chrysosporium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.B4 glycerol + O2 17% activity compared to methanol Phanerodontia chrysosporium glyceraldehyde + H2O2
-
 ?
1.1.3.13 1-propanol + O2 27% activity compared to methanol Phanerodontia chrysosporium n-propanal + H2O2
-
 ?
1.1.3.13 1-propanol + O2 27% activity compared to methanol Phanerodontia chrysosporium DSMZ 1547 n-propanal + H2O2
-
 ?
1.1.3.13 ethanol + O2 60% activity compared to methanol Phanerodontia chrysosporium acetaldehyde + H2O2
-
 ?
1.1.3.13 ethanol + O2 60% activity compared to methanol Phanerodontia chrysosporium DSMZ 1547 acetaldehyde + H2O2
-
 ?
1.1.3.13 glycerol + O2 17% activity compared to methanol Phanerodontia chrysosporium glyceraldehyde + H2O2
-
 ?
1.1.3.13 glycerol + O2 17% activity compared to methanol Phanerodontia chrysosporium DSMZ 1547 glyceraldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 100% activity Phanerodontia chrysosporium formaldehyde + H2O2
-
 ?
1.1.3.13 methanol + O2 100% activity Phanerodontia chrysosporium DSMZ 1547 formaldehyde + H2O2
-
 ?
1.1.3.13 n-pentanol + O2 13% activity compared to methanol Phanerodontia chrysosporium n-pentanal + H2O2
-
 ?
1.1.3.13 n-pentanol + O2 13% activity compared to methanol Phanerodontia chrysosporium DSMZ 1547 n-pentanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.B4 homodimer 2 * 75000, SDS-PAGE Phanerodontia chrysosporium
1.1.3.13 homodimer 2 * 75000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOX
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.B4 50
-
-
 Phanerodontia chrysosporium
1.1.3.13 50
-
-
 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.B4 9
-
-
 Phanerodontia chrysosporium
1.1.3.13 9
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.B4 7 10 more than 60% activity between pH 7.0 and 10.0 Phanerodontia chrysosporium
1.1.3.13 7 10 more than 60% activity between pH 7.0 and 10.0 Phanerodontia chrysosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.B4 Phanerodontia chrysosporium isoelectric focusing
-
 5.4
1.1.3.13 Phanerodontia chrysosporium isoelectric focusing
-
 5.4