EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.15.19 | pharmacology | modification at the C-1 position of a steroid is of pharmaceutical interest. Biotransformation can overcome tedious multistep chemical synthesis | Sorangium cellulosum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.19 | expression in a soluble form in Escherichia coli | Sorangium cellulosum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.15.19 | 0.031 | - |
androstenedione | pH and temperature not specified in the publication | Sorangium cellulosum | |
1.14.15.19 | 0.04 | - |
testosterone | pH and temperature not specified in the publication | Sorangium cellulosum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.15.19 | 50220 | - |
ESI-TOF mass spectrometry, holoenzyme | Sorangium cellulosum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.19 | Sorangium cellulosum | A9FDB7 | Soce56 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.19 | - |
Sorangium cellulosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.19 | 11-oxoandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxy-11-oxoandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
1.14.15.19 | androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxyandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
1.14.15.19 | testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
1.14.15.19 | testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | Escherichia coli-based whole-cell biocatalyst system produces a mixture (1:2.5:3) of an unknown compound and 1alpha- and 9alpha-hydroxytestosterone acetate | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.19 | CYP260A1 | - |
Sorangium cellulosum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.19 | cytochrome P-450 | cytochrome P-450-dependent monooxygenase | Sorangium cellulosum |