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Literature summary extracted from

  • Drees, S.L.; Fetzner, S.
    PqsE of Pseudomonas aeruginosa acts as pathway-specific thioesterase in the biosynthesis of alkylquinolone signaling molecules (2015), Chem. Biol., 22, 611-618.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.32 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.32 0.8
-
 cysteamine S-phosphate pH 8.2, 30°C Pseudomonas aeruginosa
3.1.2.32 2.5
-
 acetoacetyl-CoA pH 8.2, 30°C Pseudomonas aeruginosa
3.1.2.32 4.4
-
 S-ethyl-acetothioacetate pH 8.2, 30°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.32 2-aminobenzoylacetyl-CoA + H2O Pseudomonas aeruginosa
-
 2-aminobenzoylacetate + CoA
-
 ?
3.1.2.32 2-aminobenzoylacetyl-CoA + H2O Pseudomonas aeruginosa UCBPP-PA14
-
 2-aminobenzoylacetate + CoA
-
 ?
3.1.2.32 additional information Pseudomonas aeruginosa no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA ?
-
 ?
3.1.2.32 additional information Pseudomonas aeruginosa UCBPP-PA14 no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.262 Pseudomonas aeruginosa P20582 cf. EC 2.3.1.230
-
3.1.2.32 Pseudomonas aeruginosa A0A0H2Z6F6
-
-
3.1.2.32 Pseudomonas aeruginosa UCBPP-PA14 A0A0H2Z6F6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.32 2-aminobenzoylacetyl-CoA + H2O
-
 Pseudomonas aeruginosa 2-aminobenzoylacetate + CoA
-
 ?
3.1.2.32 2-aminobenzoylacetyl-CoA + H2O
-
 Pseudomonas aeruginosa UCBPP-PA14 2-aminobenzoylacetate + CoA
-
 ?
3.1.2.32 acetoacetyl-CoA + H2O
-
 Pseudomonas aeruginosa 3-oxobutanoic acid + CoA
-
 ?
3.1.2.32 acetoacetyl-CoA + H2O
-
 Pseudomonas aeruginosa UCBPP-PA14 3-oxobutanoic acid + CoA
-
 ?
3.1.2.32 cysteamine S-phosphate + H2O
-
 Pseudomonas aeruginosa cysteamine + phosphate
-
 ?
3.1.2.32 cysteamine S-phosphate + H2O
-
 Pseudomonas aeruginosa UCBPP-PA14 cysteamine + phosphate
-
 ?
3.1.2.32 additional information no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA Pseudomonas aeruginosa ?
-
 ?
3.1.2.32 additional information no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA Pseudomonas aeruginosa UCBPP-PA14 ?
-
 ?
3.1.2.32 S-ethyl-acetothioacetate + H2O
-
 Pseudomonas aeruginosa 3-oxobutanoic acid + thioethanol
-
 ?
3.1.2.32 S-ethyl-acetothioacetate + H2O
-
 Pseudomonas aeruginosa UCBPP-PA14 3-oxobutanoic acid + thioethanol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.32 0.022
-
 cysteamine S-phosphate pH 8.2, 30°C Pseudomonas aeruginosa
3.1.2.32 0.36
-
 acetoacetyl-CoA pH 8.2, 30°C Pseudomonas aeruginosa
3.1.2.32 0.85
-
 S-ethyl-acetothioacetate pH 8.2, 30°C Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.3.1.262 physiological function in addition to PqsABCD, PqsE has a role in 2-heptyl-4(1H)-quinolone synthesis. PqsE acts as thioesterase, hydrolyzing the biosynthetic intermediate 2-aminobenzoylacetyl-coenzyme A formed by PsqD to form 2-aminobenzoylacetate, the precursor of 2-heptyl-4(1H)-quinolone and 2-aminoacetophenone Pseudomonas aeruginosa
3.1.2.32 physiological function in biosynthesis of the alkylquinolones 2-heptyl-3-hydroxy-4(1H)-quinolone and 2-heptyl-4(1H)-quinolone, PqsE acts as thioesterase, hydrolyzing the biosynthetic intermediate 2-aminobenzoylacetyl-coenzyme A to form 2-aminobenzoylacetate, the precursor of 2-heptyl-4(1H)-quinolone and 2-aminoacetophenone. The role of PqsE can be taken over to some extent by the broad-specificity thioesterase TesB. A pqsE mutant produces increased levels of 2,4-dihydroxyquinoline, resulting from intramolecular cyclization of 2-aminobenzoylacetyl-coenzyme A Pseudomonas aeruginosa