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Literature summary extracted from

  • Wang, L.; Lu, Z.; Allen, K.; Mariano, P.; Dunaway-Mariano, D.
    Human symbiont Bacteroides thetaiotaomicron synthesizes 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) (2008), Chem. Biol., 15, 893-897.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.132 0.11
-
 phosphoenolpyruvate pH 7.5, 25°C Bacteroides thetaiotaomicron
2.5.1.132 1.4
-
 D-mannose 6-phosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
2.7.7.92 0.51
-
 CTP pH 7.5, 25°C Bacteroides thetaiotaomicron
2.7.7.92 1.04
-
 3-deoxy-D-glycero-D-galacto-nononate pH 7.5, 25°C Bacteroides thetaiotaomicron
3.1.3.103 0.11
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate pH 7, 25°C Bacteroides thetaiotaomicron
3.1.3.103 0.12
-
 N-acetylneuraminic acid 9-phosphate pH 7, 25°C Bacteroides thetaiotaomicron
3.1.3.103 0.31
-
 2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate pH 7, 25°C Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O Bacteroides thetaiotaomicron the 3-deoxy-D-glycero-D-galacto-nononate unit might be used to protect the cell from bacteriophage invasion and/or to mimic the human epithelial cell surface 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 r
3.1.3.103 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O Bacteroides thetaiotaomicron
-
 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?
3.1.3.103 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O Bacteroides thetaiotaomicron DSM 2079
-
 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.132 Bacteroides thetaiotaomicron Q8A711
-
-
2.7.7.92 Bacteroides thetaiotaomicron Q8A710
-
-
2.7.7.92 Bacteroides thetaiotaomicron DSM 2079 Q8A710
-
-
3.1.3.103 Bacteroides thetaiotaomicron Q8A712
-
-
3.1.3.103 Bacteroides thetaiotaomicron DSM 2079 Q8A712
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O the 3-deoxy-D-glycero-D-galacto-nononate unit might be used to protect the cell from bacteriophage invasion and/or to mimic the human epithelial cell surface Bacteroides thetaiotaomicron 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 r
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O the enzyme is specific for mannose 6-phosphate Bacteroides thetaiotaomicron 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 r
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Bacteroides thetaiotaomicron diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Bacteroides thetaiotaomicron DSM 2079 diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
3.1.3.103 2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate + H2O
-
 Bacteroides thetaiotaomicron 2-dehydro-3-deoxy-D-manno-octulosonic acid + phosphate
-
 ?
3.1.3.103 2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 2-dehydro-3-deoxy-D-manno-octulosonic acid + phosphate
-
 ?
3.1.3.103 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?
3.1.3.103 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?
3.1.3.103 N-acetylneuraminic acid 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron N-acetylneuraminic acid + phosphate
-
 ?
3.1.3.103 N-acetylneuraminic acid 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 N-acetylneuraminic acid + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.132 BT1714
-
 Bacteroides thetaiotaomicron
2.5.1.132 KDN-9P synthase
-
 Bacteroides thetaiotaomicron
2.7.7.92 BT_1715
-
 Bacteroides thetaiotaomicron
3.1.3.103 BT_1713
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.132 25
-
 assay at Bacteroides thetaiotaomicron

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.132 0.017
-
 phosphoenolpyruvate pH 7.5, 25°C Bacteroides thetaiotaomicron
2.5.1.132 0.017
-
 D-mannose 6-phosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
2.7.7.92 1.02
-
 CTP pH 7.5, 25°C Bacteroides thetaiotaomicron
3.1.3.103 0.06
-
 2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate pH 7, 25°C Bacteroides thetaiotaomicron
3.1.3.103 0.73
-
 N-acetylneuraminic acid 9-phosphate pH 7, 25°C Bacteroides thetaiotaomicron
3.1.3.103 1.2
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate pH 7, 25°C Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.132 7.5
-
 assay at Bacteroides thetaiotaomicron

General Information

EC Number General Information Comment Organism
2.7.7.92 physiological function enzyme is part of a biosynthetic pathway that forms and cytidylates 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid, most likely for inclusion in the capsular polysaccharides encoded by Bacteroides thetaiotaomicron CP locus 7 Bacteroides thetaiotaomicron
3.1.3.103 physiological function enzyme belongs to a biosynthetic pathway that forms and cytidylates 2-dehydro-3-deoxy-D-glycero-D-galacto-nononic acid Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.132 0.012
-
 D-mannose 6-phosphate pH 7.5, 25°C Bacteroides thetaiotaomicron
2.5.1.132 0.15
-
 phosphoenolpyruvate pH 7.5, 25°C Bacteroides thetaiotaomicron