Literature summary extracted from
Kuhn, C.D.; Wilusz, J.E.; Zheng, Y.; Beal, P.A.; Joshua-Tor, L.
On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme (2015), Cell, 160, 644-658.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.7.72 |
- |
Archaeoglobus fulgidus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.7.72 |
in complex with a human MenBeta minihelix. The unstable minihelix is bound between the enzymes catalytic center, comprised of the head and neck domains, and its tail domain. The minihelix perfectly mimics full-length tRNA with its acceptor and TPsiC stems folding into a continuous A-type RNA helix. The TPsiC loop is in the same conformation as in full-length tRNA |
Archaeoglobus fulgidus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.7.72 |
Archaeoglobus fulgidus |
O28126 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.7.72 |
additional information |
tRNA minihelices, which contain only the acceptor stem and TPsiC stem-loop, are also efficiently subjected to CCACCA addition when they have guanosines at the first and second positions as well as a destabilized acceptor stem by virtue of mismatches and G-U wobbles |
Archaeoglobus fulgidus |
? |
- |
? |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.7.7.72 |
physiological function |
whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates |
Archaeoglobus fulgidus |