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Literature summary extracted from

  • Merkulova, N.; Sedova, V.
    Modified in vivo subunits of DNA-dependent RNA polymerase III in mammals (2015), Cell Tissue Biol., 9, 284-289.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.6 nucleus
-
 Mus musculus 5634
-
2.7.7.6 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ required Mus musculus
2.7.7.6 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Mus musculus
-
 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Homo sapiens
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Homo sapiens
-
-
-
2.7.7.6 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.6 glycoprotein the three subunits are simultaneously glycosylated in vivo by O-N-acetylglucosamine residues, glycosylation and phosphorylation of the three subunits is dynamic and differs between the subunits, , also depending on the physiological state of the cell, overview Homo sapiens
2.7.7.6 glycoprotein the two subunits are simultaneously glycosylated by N-O-acetylglucosamine residues in vivo, glycosylation and phosphorylation of the two subunits is dynamic and differs between the subunits, , also depending on the physiological state of the cell, overview Mus musculus
2.7.7.6 phosphoprotein the three subunits are simultaneously phosphorylated on serine and threonine residues in vivo, glycosylation and phosphorylation of the three subunits is dynamic and differs between the subunits, , also depending on the physiological state of the cell, overview Homo sapiens
2.7.7.6 phosphoprotein the two subunits are simultaneously phosphorylated on serine and threonine residues in vivo, glycosylation and phosphorylation of the three subunits is dynamic and differs between the subunits, also depending on the physiological state of the cell, overview Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 RNA polymerase III subunits isolation from A-431 cells and placenta cell nuclei by anion exchange chromatography and ultrafiltration Homo sapiens
2.7.7.6 RNA polymerase III subunits isolation from fibroblast nuclei by anion exchange chromatography and ultrafiltration Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.6 3T3 cell
-
 Mus musculus
-
2.7.7.6 A-431 cell
-
 Homo sapiens
-
2.7.7.6 epidermoid carcinoma cell
-
 Homo sapiens
-
2.7.7.6 fibroblast
-
 Mus musculus
-
2.7.7.6 placenta mature Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Mus musculus diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn
-
 Homo sapiens diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 More subunits with molecular weights of 49 kDa and 42 kDa and a subunit with a molecular weight of 45 kDa (which is probably a component of the basal transcription factor of RNA polymerase III, since it is not identified in the mouse enzyme) are modified in the composition of the enzyme isolated from mouse fibroblasts. The two subunits are simultaneously phosphorylated and glycosylated (glycosylation by O-N-acetylglucosamine residues) Mus musculus
2.7.7.6 More subunits with molecular weights of 60 kDa and 45 kDa and a subunit with a molecular weight of 52 kDa (which is probably one of the basal transcription factors of RNA polymerase III) are modified in the composition of the enzyme isolated from human cells. The three subunits are simultaneously phosphorylated and glycosylated Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase III
-
 Mus musculus
2.7.7.6 RNA polymerase III
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
 assay at Mus musculus
2.7.7.6 7.9
-
 assay at Homo sapiens