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Literature summary extracted from

  • Tsao, N.; Lee, M.H.; Zhang, W.; Cheng, Y.C.; Chang, Z.F.
    The contribution of CMP kinase to the efficiency of DNA repair (2015), Cell Cycle, 14, 354-363.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.14 additional information enzyme knockdown in MCF-7 cells using a CMPK shRNA lentivirus Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.14 nucleus localization of enzyme CMPK at DNA damage sites, co-localization and complex formation with Tip60 and ribonucleotide reductase Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.14 ATP + CMP Homo sapiens
-
ADP + CDP
-
?
2.7.4.14 ATP + UMP Homo sapiens
-
ADP + UDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Homo sapiens P30085 gene CMPK1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 breast adenocarcinoma cell
-
Homo sapiens
-
2.7.4.14 MCF-7 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + CMP
-
Homo sapiens ADP + CDP
-
?
2.7.4.14 ATP + CMP preferred substrate Homo sapiens ADP + CDP
-
?
2.7.4.14 ATP + dCMP
-
Homo sapiens ADP + dCDP
-
?
2.7.4.14 ATP + dUMP
-
Homo sapiens ADP + dUDP
-
?
2.7.4.14 ATP + UMP
-
Homo sapiens ADP + UDP
-
?
2.7.4.14 ATP + UMP preferred substrate Homo sapiens ADP + UDP
-
?
2.7.4.14 additional information CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.14 CMP kinase
-
Homo sapiens
2.7.4.14 CMP/UMP kinase
-
Homo sapiens
2.7.4.14 CMPK
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.14 ATP
-
Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.4.14 malfunction knockdown of CMPK delays DNA repair during recovery from UV damage in serum-deprived cells but not in the cells without serum deprivation. Exogenous supply of cytidine or deoxycytidine facilitates DNA repair dependent on CMPK in serum-deprived cells. But CMPK knockdown does not affect the steady state level of dCTP in serum-deprived cells. Re-expression of wild-type but not N-terminus deleted CMPK restores the efficiency of DNA repair in CMPK knockdown cells Homo sapiens
2.7.4.14 additional information the N-terminal region of CMPK is essential for the complex formation and recruitment to DNA damage sites Homo sapiens
2.7.4.14 physiological function the enzyme enzyme catalyzes CDP formation in DNA repair, the synthesis of dCDP or CDP determines the rate of repair. The N-terminal 32-amino-acid of enzyme CMPK is required for its recruitment to DNA damage sites in a Tip60-dependent manner, complex of CMPK/RNR/Tip60 and the recruitment to the sites of DNA damage, overview. Site-specific dCDP formation via CMPK provides a means to facilitate DNA repair in serum-deprived cells, analysis of functional contribution of CMPK to dCTP pool and the rate of DNA compared in MCF-7 cells in serum-containing and serum-deprived conditions Homo sapiens