BRENDA - Enzyme Database

The contribution of CMP kinase to the efficiency of DNA repair

Tsao, N.; Lee, M.H.; Zhang, W.; Cheng, Y.C.; Chang, Z.F.; Cell Cycle 14, 354-363 (2015)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
additional information
enzyme knockdown in MCF-7 cells using a CMPK shRNA lentivirus
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
nucleus
localization of enzyme CMPK at DNA damage sites, co-localization and complex formation with Tip60 and ribonucleotide reductase
Homo sapiens
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
P30085
gene CMPK1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
breast adenocarcinoma cell
-
Homo sapiens
-
2.7.4.14
MCF-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
737991
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
preferred substrate
737991
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
737991
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
737991
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
-
737991
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
preferred substrate
737991
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP
737991
Homo sapiens
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.14
additional information
enzyme knockdown in MCF-7 cells using a CMPK shRNA lentivirus
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
nucleus
localization of enzyme CMPK at DNA damage sites, co-localization and complex formation with Tip60 and ribonucleotide reductase
Homo sapiens
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
breast adenocarcinoma cell
-
Homo sapiens
-
2.7.4.14
MCF-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
737991
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
preferred substrate
737991
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
737991
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
737991
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
-
737991
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
preferred substrate
737991
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP
737991
Homo sapiens
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
2.7.4.14
malfunction
knockdown of CMPK delays DNA repair during recovery from UV damage in serum-deprived cells but not in the cells without serum deprivation. Exogenous supply of cytidine or deoxycytidine facilitates DNA repair dependent on CMPK in serum-deprived cells. But CMPK knockdown does not affect the steady state level of dCTP in serum-deprived cells. Re-expression of wild-type but not N-terminus deleted CMPK restores the efficiency of DNA repair in CMPK knockdown cells
Homo sapiens
2.7.4.14
additional information
the N-terminal region of CMPK is essential for the complex formation and recruitment to DNA damage sites
Homo sapiens
2.7.4.14
physiological function
the enzyme enzyme catalyzes CDP formation in DNA repair, the synthesis of dCDP or CDP determines the rate of repair. The N-terminal 32-amino-acid of enzyme CMPK is required for its recruitment to DNA damage sites in a Tip60-dependent manner, complex of CMPK/RNR/Tip60 and the recruitment to the sites of DNA damage, overview. Site-specific dCDP formation via CMPK provides a means to facilitate DNA repair in serum-deprived cells, analysis of functional contribution of CMPK to dCTP pool and the rate of DNA compared in MCF-7 cells in serum-containing and serum-deprived conditions
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.4.14
malfunction
knockdown of CMPK delays DNA repair during recovery from UV damage in serum-deprived cells but not in the cells without serum deprivation. Exogenous supply of cytidine or deoxycytidine facilitates DNA repair dependent on CMPK in serum-deprived cells. But CMPK knockdown does not affect the steady state level of dCTP in serum-deprived cells. Re-expression of wild-type but not N-terminus deleted CMPK restores the efficiency of DNA repair in CMPK knockdown cells
Homo sapiens
2.7.4.14
additional information
the N-terminal region of CMPK is essential for the complex formation and recruitment to DNA damage sites
Homo sapiens
2.7.4.14
physiological function
the enzyme enzyme catalyzes CDP formation in DNA repair, the synthesis of dCDP or CDP determines the rate of repair. The N-terminal 32-amino-acid of enzyme CMPK is required for its recruitment to DNA damage sites in a Tip60-dependent manner, complex of CMPK/RNR/Tip60 and the recruitment to the sites of DNA damage, overview. Site-specific dCDP formation via CMPK provides a means to facilitate DNA repair in serum-deprived cells, analysis of functional contribution of CMPK to dCTP pool and the rate of DNA compared in MCF-7 cells in serum-containing and serum-deprived conditions
Homo sapiens